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We obtained <jats:italic>S. fradiae\u2010<\/jats:italic>nitR<jats:sup>+<\/jats:sup>bld, a nitrone\u2010oligomycin A resistant mutant with a \u00abbald\u00bb phenotype. Comparative genomic analysis of the wild\u2010type <jats:italic>S. fradiae<\/jats:italic> ATCC19609 and <jats:italic>S. fradiae<\/jats:italic>\u2010nitR<jats:sup>+<\/jats:sup>bld revealed a mutation in <jats:italic>pa<\/jats:italic>dR \u2013 a gene encoding a multifunction transcription regulator, which resulted in the amino acid replacement in a highly conserved DNA\u2010binding domain. Bioinformatics genome analysis of <jats:italic>S. fradiae<\/jats:italic> ATCC19609 discovered a PadR binding site 13\u2009bp upstream the start codon of the <jats:italic>marR<\/jats:italic> transcription factor gene. Induction of <jats:italic>S. fradiae<\/jats:italic>nitR<jats:sup>+<\/jats:sup>bld and w.t. strains with nitrone\u2010oligomycin A lead to a significant increase in expression level of the <jats:italic>marR<\/jats:italic> gene in the w.t. strain, but no change observed in mutant strain. We identified differences between DNA\u2013protein interactions of the mutant and native PadR proteins with its putative binding site in <jats:italic>S. fradiae<\/jats:italic> ATCC19609. This allowed us to suggest that the <jats:italic>pa<\/jats:italic>dR gene, that harbored a single nucleotide mutation in the <jats:italic>S. fradiae<\/jats:italic>nitR<jats:sup>+<\/jats:sup>bld strain, might be involved in the mechanism of resistance to nitrone\u2010oligomycin A. We assume the participation of the transcriptional factor<jats:italic>pa<\/jats:italic>dR in the formation of the bald phenotype.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201800095","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T12:34:08Z","timestamp":1530534848000},"page":"739-746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A functional study of the global transcriptional regulator PadR from a strain <i>Streptomyces fradiae<\/i>\u2010nitR+bld, resistant to nitrone\u2010oligomycin"],"prefix":"10.1002","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6499-3908","authenticated-orcid":false,"given":"Aleksey A.","family":"Vatlin","sequence":"first","affiliation":[{"name":"Vavilov Institute of General Genetics Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga 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