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One of the main structures for T6SS is Hcp (hemolysin co\u2010regulation protein) pipeline. To investigate the role of Hcp major sub\u2010unit genes <jats:italic>hcp<jats:sub>1<\/jats:sub><\/jats:italic> and <jats:italic>hcp<jats:sub>2<\/jats:sub><\/jats:italic>, we deleted <jats:italic>hcp<jats:sub>1<\/jats:sub><\/jats:italic> and <jats:italic>hcp<jats:sub>2<\/jats:sub><\/jats:italic> genes for constructing the in\u2010frame gene deletion mutants. The properties of biofilm formation and the adhesion to chicken embryo fibroblasts cells (DF1 cells) were reduced in the <jats:italic>hcp<jats:sub>2<\/jats:sub><\/jats:italic> mutant. The knockout of <jats:italic>hcp<jats:sub>1<\/jats:sub><\/jats:italic> and <jats:italic>hcp<\/jats:italic><jats:sub>2<\/jats:sub> genes reduced the ability of the avian pathogenic <jats:italic>Escherichia coli<\/jats:italic> (APEC) strain CE129 to infect developing chicken embryos. The expression of quorum sensing (QS)\u2010associated genes <jats:italic>luxS<\/jats:italic>, <jats:italic>lsrR<\/jats:italic>, and <jats:italic>pfs<\/jats:italic> were down\u2010regulated in the <jats:italic>hcp<jats:sub>1<\/jats:sub><\/jats:italic> mutant, and the expression of type 1 fimbriae gene <jats:italic>fimA<\/jats:italic> and the adhesion\u2010related genes <jats:italic>fimC<\/jats:italic> and <jats:italic>papC<\/jats:italic> were decreased in the <jats:italic>hcp<jats:sub>2<\/jats:sub><\/jats:italic> mutant, as well as the expression of anti\u2010serum survival factor genes <jats:italic>ompA<\/jats:italic> and <jats:italic>iss<\/jats:italic> were inhibited in both <jats:italic>hcp<jats:sub>1<\/jats:sub><\/jats:italic> and <jats:italic>hcp<jats:sub>2<\/jats:sub><\/jats:italic> mutants. These results described above from this study help to further elaborate the role of HCP in APEC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201800156","type":"journal-article","created":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T14:29:14Z","timestamp":1535984954000},"page":"938-946","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The different roles of <i>hcp<sub>1<\/sub><\/i> and <i>hcp<sub>2<\/sub><\/i> of the type VI secretion system in <i>Escherichia coli<\/i> strain CE129"],"prefix":"10.1002","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1238-0621","authenticated-orcid":false,"given":"Xueyan","family":"Ding","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Important Animal Infectious Diseases and Zoonoses Yangzhou China"},{"name":"Joint International Research Laboratory of 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