{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T21:07:36Z","timestamp":1781730456754,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>A biosurfactant, produced by <jats:italic>Achromobacter<\/jats:italic> sp. TMB1 is reported through this investigation having physiochemical properties useful for operational and remedial activities in petroleum industries. The strain isolated from soils of local petrol pumps when allowed to grow in glucose containing mineral salts media (MSM) in ambient environment that is, 30\u2009\u00b0C and varying pH in between 5.5 and 7.2, showed surface tension reduction as low as 34.25\u2009dyne\u2009cm<jats:sup>\u22121<\/jats:sup> at an interval of 48\u201372\u2009h. Taguchi experiment confirmed the contribution of glucose, yeast extracts, and NaNO<jats:sub>3<\/jats:sub> concentration on biosurfactant production. FTIR and <jats:sup>1<\/jats:sup>H NMR analysis of culture supernatant obtained after acid precipitation and solvent extraction methods revealed the glycolipid nature of the extracted compounds. HR\u2010LCMS study further revealed the presence of 10 different types of mono\u2010 and di\u2010rhamnolipids congeners with the fatty acids carbon length C<jats:sub>8\u201312<\/jats:sub>. The isolated biosurfactant shows functional stability in the temperature range 20\u2013100\u2009\u00b0C and pH range 2\u201312 while maintaining the structural integrity till 550\u2009\u00b0C (TGA\u2010DSC), strongly suggests its potential applications in petroleum industries.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201800298","type":"journal-article","created":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T08:39:39Z","timestamp":1546418379000},"page":"238-248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Optimization and characterization of a glycolipid produced by <i>Achromobacter<\/i> sp. to use in petroleum industries"],"prefix":"10.1002","volume":"59","author":[{"given":"Saurav","family":"Haloi","sequence":"first","affiliation":[{"name":"Applied Biochemistry Lab Department of Molecular Biology and Biotechnology Tezpur University Napaam, Sonitpur Assam 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