{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:09:25Z","timestamp":1780636165047,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800126"],"award-info":[{"award-number":["31800126"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium (<jats:italic>S<\/jats:italic>. Tm) is a major intracellular pathogen that infects humans and animals, and its survival and growth in macrophages is essential for its pathogenicity. More than 50 putative regulatory proteins are encoded by the <jats:italic>S<\/jats:italic>. Tm genome, but the functions of these regulatory proteins in mediating <jats:italic>S<\/jats:italic>. Tm pathogenicity are largely unknown. In this study, we investigated the biological function of the <jats:italic>STM0030<\/jats:italic> gene, which encodes a putative LysR\u2010type transcriptional regulator. We found that <jats:italic>STM0030<\/jats:italic> is upregulated 2.8\u20135.7\u2010fold during <jats:italic>S<\/jats:italic>. Tm growth in macrophages. Further, mutating this gene decreased bacterial growth in macrophages and attenuated virulence in mice. RNA\u2010sequencing to investigate the regulatory function of <jats:italic>STM0030<\/jats:italic> in <jats:italic>S<\/jats:italic>. Tm revealed that 447 genes were differentially expressed between the mutant and the wild\u2010type strains; 429 of these genes were downregulated, suggesting that STM0030 mainly acts as a transcriptional activator. Moreover, the expression of gluconate, maltose, and hexose\u2010p transport genes, as well as allantoin utilization genes were downregulated in the <jats:italic>STM0030<\/jats:italic> mutant; this might be associated with the observed decrease in intracellular replication and pathogenicity of the mutant. Our findings suggest that STM0030 is a new pathogenicity\u2010associated regulatory protein that broadens our understanding of the virulence regulatory network of <jats:italic>S<\/jats:italic>. Tm.<\/jats:p>","DOI":"10.1002\/jobm.201900315","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T13:09:09Z","timestamp":1570021749000},"page":"1143-1153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The LysR\u2010type transcriptional regulator STM0030 contributes to <i>Salmonella<\/i> Typhimurium growth in macrophages and virulence in mice"],"prefix":"10.1002","volume":"59","author":[{"given":"Huan","family":"Zhang","sequence":"first","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin China"},{"name":"Key Laboratory of Microbial Functional Genomics  Tianjin China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University  Tianjin China"},{"name":"College of Life Sciences Nankai University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorui","family":"Song","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin China"},{"name":"Key Laboratory of Microbial Functional Genomics  Tianjin China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University  Tianjin China"},{"name":"College of Life Sciences Nankai University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runxia","family":"Lv","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin China"},{"name":"Key Laboratory of Microbial Functional Genomics  Tianjin China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqian","family":"Liu","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin China"},{"name":"Key Laboratory of Microbial Functional Genomics  Tianjin China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peisheng","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin China"},{"name":"Key Laboratory of Microbial Functional Genomics  Tianjin China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University  Tianjin China"},{"name":"College of Life Sciences Nankai University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8580-606X","authenticated-orcid":false,"given":"Lingyan","family":"Jiang","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology Nankai University  Tianjin China"},{"name":"Key Laboratory of Microbial Functional Genomics  Tianjin China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education Nankai University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,2]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.P11101"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1203.050854"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/318457"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2008.03.012"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100007"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00066-12"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00137.x"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2128"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.058115-0"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110225"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00023-07"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801872105"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02051-15"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006429"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M117.068296"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2018.03.003"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03313.x"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404299200"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.2.237"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00896"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5334.2007"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404299200"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12628"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527682386.ch3"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-011-0034-0"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.05.006"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.47.100193.003121"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.5.1285-1291.1995"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.12.033"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01484-14"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.19.5539-5550.2000"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003301"},{"key":"e_1_2_8_1_37_1","first-page":"4","article-title":"Salmonella\u2014how a metabolic generalist adopts an intracellular lifestyle during infection","author":"Dandekar T","year":"2015","journal-title":"Front Cell Infect Microbiol"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101869"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.02.001"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjobm.201900315","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.201900315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T08:09:25Z","timestamp":1694246965000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.201900315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,2]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/jobm.201900315"],"URL":"https:\/\/doi.org\/10.1002\/jobm.201900315","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,2]]},"assertion":[{"value":"2019-06-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}