{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:09:26Z","timestamp":1790399366083,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T00:00:00Z","timestamp":1574035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["C2019407070"],"award-info":[{"award-number":["C2019407070"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802186"],"award-info":[{"award-number":["31802186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2017M611935"],"award-info":[{"award-number":["2017M611935"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Salmonella<\/jats:italic> Enteritidis is an important foodborne pathogen that can infect a wide range of animal species including human beings, resulting in great losses to commercial husbandry and human health. CirA is an outer membrane receptor involved in iron uptake and colicin1A\/B\u2010mediated competitive killing. Although iron uptake is crucial to bacterial virulence, limited literature is available about the role of CirA in infection. In the present work, we aimed to evaluate the role of CirA during <jats:italic>S.<\/jats:italic> Enteritidis infection. For this purpose, we generated a CirA\u2010deficient mutant of the\u00a0<jats:italic>S.<\/jats:italic> Enteritidis strain C50336 and examined its biological characteristics. The results showed that <jats:italic>cirA<\/jats:italic> gene inactivation caused sharply decreased biofilm formation and apparently impaired antibiotic resistance. Furthermore, the <jats:italic>cirA<\/jats:italic> gene deletion mutant showed markedly reduced adhesion and invasion to human epithelial cell line Caco\u20102 cells and decreased proliferation in mouse macrophage cell line RAW264.7 cells. Moreover, attenuated virulence was determined by a mouse model, with an LD<jats:sub>50<\/jats:sub> increase of approximately 1,000\u2010fold. These data indicated that CirA plays critical roles in the <jats:italic>S.<\/jats:italic> Enteritidis infection process.<\/jats:p>","DOI":"10.1002\/jobm.201900418","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T10:27:40Z","timestamp":1574072860000},"page":"72-81","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Contribution of the colicin receptor CirA to biofilm formation, antibotic resistance, and pathogenicity of <i>Salmonella<\/i> Enteritidis"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-3212","authenticated-orcid":false,"given":"Zhiqiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Hebei Key Laboratory of Preventive Veterinary Medicine Hebei Normal University of Science and Technology  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wannian","family":"Du","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Preventive Veterinary Medicine Hebei Normal University of Science and Technology  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Preventive Veterinary Medicine Hebei Normal University of Science and Technology  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Li","sequence":"additional","affiliation":[{"name":"The Second Hospital of Qinhuangdao  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuoqing","family":"Su","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Preventive Veterinary Medicine Hebei Normal University of Science and Technology  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonglei","family":"Wu","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Preventive Veterinary Medicine Hebei Normal University of Science and Technology  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhuan","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agriculture University  Changchun Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agriculture University  Changchun Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiumei","family":"Shi","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Preventive Veterinary Medicine Hebei Normal University of Science and Technology  Qinhuangdao Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Co\u2010Innovation Center for Important Animal Infectious Diseases and Zoonoses, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety of Ministry of Education of China, College of Veterinary Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.046185-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2017.02.010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1111818"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/1592416"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2002.tb00606.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.10.025"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140510608"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005207"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.08.006"},{"key":"e_1_2_7_12_1","first-page":"5","article-title":"Dihydroxybenzolyserine\u2010a siderophore for E. coli","volume":"67","author":"Hantke K","year":"1990","journal-title":"FEMS Microbiol Lett"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003844"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31876"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.05.015"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029968"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2014.10.003"},{"key":"e_1_2_7_20_1","first-page":"6072","article-title":"Iron regulatory proteins mediate host resistance to Salmonella infection","volume":"118","author":"Nairz M","year":"2016","journal-title":"HHS Public Access"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00173"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035914"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03407-14"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00241"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.3.1163-1168.2005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2003.08.005"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2015.01.004"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0597-8"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01824.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00481"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2012.00332.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02882-14"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.020"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6953-6961.2003"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjobm.201900418","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.201900418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T17:31:00Z","timestamp":1694107860000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.201900418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,18]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1002\/jobm.201900418"],"URL":"https:\/\/doi.org\/10.1002\/jobm.201900418","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,18]]},"assertion":[{"value":"2019-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}