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The collected milk samples were initially screened by a <jats:italic>Brucella<\/jats:italic> selective medium. The bacterial isolates grown on the selective medium were subjected to biochemical identification for further confirmation of <jats:italic>Brucella<\/jats:italic> species. Among the tested samples, 28 (16.4%) were found positive for selective medium and 14 (8.2%) were found positive after biochemical confirmation. The antimicrobial susceptibility of several antibiotics performed by the disc\u2010diffusion method did not yield any significant findings. Encapsulating antimicrobial drugs in unilamellar niosomes is an effective approach to treat the endemic infection. In this study, the antimicrobial activity of niosome\u2010encapsulated levofloxacin is compared with free drug. The drug\u2010encapsulating and empty niosomes were synthesized by using two surfactants Tween 80 and Span 40. Niosomal characterization included electron microscopy, dynamic light scattering, and zeta potential. The encapsulation efficiency was found to be 78% and 74% for Span 40 and Tween 80 niosomes, respectively. The antibacterial activity of niosomal levofloxacin was evaluated against the identified <jats:italic>Brucella<\/jats:italic> species and the antimicrobial activity of the free drug was increased many folds after encapsulation. In this study, levofloxacin niosomes were successfully synthesized against Brucellosis.<\/jats:p>","DOI":"10.1002\/jobm.201900454","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T17:20:51Z","timestamp":1576776051000},"page":"281-290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<b>Nanoniosome\u2010encapsulated levoflaxicin as an antibacterial agent against<\/b><i><b>Brucella<\/b><\/i>"],"prefix":"10.1002","volume":"60","author":[{"given":"Sana","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Biosciences COMASTS University Islamabad  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Usman","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Department of Biosciences COMASTS University Islamabad  Islamabad 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