{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:21:24Z","timestamp":1770747684870,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31602080"],"award-info":[{"award-number":["31602080"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transcriptional regulator GntR controls diverse physiological functions necessary for <jats:italic>Brucella<\/jats:italic> survival. In the intracellular pathogen <jats:italic>Brucella<\/jats:italic>, GntR has been shown to regulate the expression of genes related to virulence. However, the precise determination of GntR direct targets has so far proved elusive. Therefore, we performed chromatin immunoprecipitation of GntR10 followed by next\u2010generation sequencing (ChIP\u2010seq). We selected target gene BAB1_1163 directly regulated by GntR10 and created the mutant (2308\u03941163) from virulent <jats:italic>Brucella abortus<\/jats:italic> 2308 (S2308). 2308\u03941163 strain survival capability in murine macrophages (RAW 264.7) was detected and the levels of tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) and interleukin\u20101\u03b2 (IL\u20101\u03b2) were also measured. We detected 88 intergenic ChIP\u2010seq peaks of GntR10 binding distributed across the <jats:italic>Brucella<\/jats:italic> genome and determined a markedly asymmetric binding consensus motif with 12\u2009bp length. 2308\u03941163 showed reduced survival capability in RAW 264.7. After the macrophages were infected with 2308\u03941163, the levels of TNF\u2010\u03b1 and IL\u20101\u03b2 were decreased and were significantly lower than that for the S2308\u2010infected group, indicating that the 2308\u03941163 mutant could inhibit the secretion of inflammatory cytokines. Taken together, the research has recorded valuable data about GntR10 and provided new insights into the functionality of GntR10.<\/jats:p>","DOI":"10.1002\/jobm.201900458","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T10:26:12Z","timestamp":1573727172000},"page":"149-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["ChIP\u2010seq analysis of <i>Brucella<\/i> reveals transcriptional regulation of GntR"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-3992","authenticated-orcid":false,"given":"Zhiqiang","family":"Li","sequence":"first","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shihezi University  Shihezi 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