{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:35:33Z","timestamp":1793043333701,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses to the pork industry. The present study showed that Yansuanmalingua (YASML) can inhibit type 2 PRRSV replication using plaque assay, quantitative reverse transcriptase\u2010polymerase chain reaction, and immunofluorescence assay. Furthermore, inhibition of PRRSV replication was shown to be related to Toll\u2010like receptor 3 (TLR3)\u2010dependent apoptosis\u2010induction by YASML in the PRRSV\u2010infected MARC\u2010145, and TLR3\u2010dependent apoptosis\u2010induction by YASML was found to suppress PRRSV replication via the activation of caspase\u20108 and caspase\u20103 pathways, respectively. Meanwhile, activation of the caspase\u20103 pathway seemed to be related to the\u00a0downregulation of myeloid cell leukemia 1 (Mcl\u20101) expression. Our results showed that YASML\u2010induced TLR3\u2010dependent apoptosis could be blocked by a pan\u2010caspase inhibitor and small interfering RNA against TLR3. In conclusion, the present study demonstrates that YASML exerts its anti\u2010PRRSV effect by activating the caspase\u20108\/caspase\u20103 signaling pathway and by negatively regulating Mcl\u20101 expression. These findings not only provide new insights into the molecular mechanism of YASML inhibition of PRRSV replication via the TLR3\u2010dependent apoptosis pathway but also suggest potential, new antiviral drugs by expressing caspase\u20103 or down expressing Mcl\u20101.<\/jats:p>","DOI":"10.1002\/jobm.201900485","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T06:27:20Z","timestamp":1583130440000},"page":"400-406","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Yansuanmalingua inhibits replication of type 2 porcine reproductive and respiratory syndrome virus via activating the caspase\u20108 apoptosis pathway"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3684-8217","authenticated-orcid":false,"given":"Yaozhong","family":"Ding","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Gansu Agricultural University  Lanzhou Gansu China"},{"name":"State Key Laboratory of Veterinary Etiological Biology, National Foot\u2010and\u2010Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences  Lanzhou Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxiu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agricultural University  Lanzhou Gansu China"},{"name":"State Key Laboratory of Veterinary Etiological Biology, National Foot\u2010and\u2010Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences  Lanzhou Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzhen","family":"Cheng","sequence":"additional","affiliation":[{"name":"China Agricultural Veterinarian Biology Science and Technology Co. 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