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However, plasmids with single Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> replicons are poorly studied as a different incompatibility (Inc) group, despite their rise in appearance in some strains. Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> plasmids, pA1763\u2010KPC, and p427113\u20102, from two clinical <jats:italic>Klebsiella pneumoniae<\/jats:italic> isolates were fully sequenced by high\u2010throughput genome sequencing. Linear structural comparisons of Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> backbone region were made between these two plasmids and six arbitrarily selected representative Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> plasmids sequenced previously. A further detailed genomic comparison was carried out between plasmids pA1763\u2010KPC, p427113\u20102, and pFB2.2, which show high homology across the backbone sequence to one another. Among all sequenced Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> plasmids considered in this study, plasmids pA1763\u2010KPC and p427113\u20102 showed the most complete Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> backbones. These were composed of the Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> replicon (<jats:italic>repA<\/jats:italic><jats:sub>IncpA1763\u2010KPC<\/jats:sub> and its iterons), the 5.6\u2010kb Inc<jats:sub>pA1763\u2010KPC<\/jats:sub>\u2010type maintenance region, the 27.7\u2010kb IncFII<jats:sub>K<\/jats:sub>\u2010type maintenance region, and the 36.6\u2010kb IncFII<jats:sub>K<\/jats:sub>\u2010type conjugal transfer regions. Compared with pA1763\u2010KPC or p427113\u20102, the backbone regions of the other analyzed Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> plasmids had gradually undergone different deletions or truncations, but shared small and core Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> backbones including the Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> replicon, Inc<jats:sub>pA1763\u2010KPC<\/jats:sub>\u2010type maintenance region, and residual IncFII<jats:sub>K<\/jats:sub>\u2010type maintenance region. Accessory modules integrated into Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> backbones included the multidrug\u2010resistant module <jats:italic>bla<\/jats:italic><jats:sub>KPC\u20102<\/jats:sub> region in pA1763\u2010KPC, the metal\u2010resistance modules <jats:italic>ars<\/jats:italic> region together with <jats:italic>ncr<\/jats:italic> region in pFB2.2 and <jats:italic>sil<\/jats:italic> in pKPN\u20109a0d, the IS<jats:italic>Kpn14<\/jats:italic>\u2010to\u2010IS<jats:italic>26<\/jats:italic> region in p427113\u20102, and other non\u2010resistance region in the respective plasmids. This detailed comparative genomics analysis of Inc<jats:sub>pA1763\u2010KPC<\/jats:sub> plasmids provides a deep insight into their diversification and evolution.<\/jats:p>","DOI":"10.1002\/jobm.202000668","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T11:55:28Z","timestamp":1612871728000},"page":"219-229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparative genomic analyses of Inc<sub>pA1763\u2010KPC<\/sub> plasmids"],"prefix":"10.1002","volume":"61","author":[{"given":"Hongchao","family":"Chen","sequence":"first","affiliation":[{"name":"College of Medicine, First Affiliated Hospital Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3414-6916","authenticated-orcid":false,"given":"Zhe","family":"Zhan","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Qing","sequence":"additional","affiliation":[{"name":"College of Medicine, First Affiliated Hospital Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Mei","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongsheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Ni","sequence":"additional","affiliation":[{"name":"School of Medicine Jiangsu University  Zhenjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00838"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06283-z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S189168"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01276-14"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321364111"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02011-17"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.00800-19"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009845"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofx163.176"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40793-019-0347-1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2017.02.008"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33419"},{"key":"e_1_2_8_15_1","unstructured":"Clinical and Laboratory Standards Institute.Performance Standards for Antimicrobial Susceptibility Testing: Twenty\u2010Seventh Informational Supplement M100\u2010S28. 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