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The <jats:italic>ysnE<\/jats:italic> gene, encoding a putative tryptophan acetyltransferase, has been demonstrated to be involved in and strongly contribute to IAA production in <jats:italic>Bacillus<\/jats:italic>, but the mechanism is unknown. In this study, to investigate how <jats:italic>ysnE<\/jats:italic> participates in IAA biosynthesis in the plant growth\u2010promoting rhizobacterium <jats:italic>Bacillus amyloliquefaciens<\/jats:italic> SQR9, differences in the produced IAA biosynthesis intermediates between wild\u2010type SQR9 and \u0394ysnE were analyzed and compared, and the effects of different intermediate compounds on the production of IAA and the accumulation of other intermediates were also investigated. The results showed that the mutant \u0394ysnE produced more indole\u20103\u2010lactic acid (ILA) and tryptamine (TAM) than the SQR9 wild\u2010type strain (nearly 1.6\u2010 and 2.1\u2010fold), while the production of tryptophol (TOL) was significantly decreased by 46%. When indole\u20103\u2010pyruvic acid (IPA) served as the substrate, the concentration of ILA in the \u0394ysnE fermentation broth was much higher than that of the wild type, while IAA and TOL were significantly lower, and \u0394ysnE was lower than SQR9 in IAA and TOL with the addition of TAM. The TOL content in the \u0394ysnE fermentation broth was much lower than that in the wild\u2010type SQR9 with the addition of ILA. We suggest that <jats:italic>ysnE<\/jats:italic> may be involved in the IPA and TAM pathways and play roles in indole acetaldehyde (IAAld) synthesis from IPA and TAM and in the conversion of ILA to TOL.<\/jats:p>","DOI":"10.1002\/jobm.202100098","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T19:00:01Z","timestamp":1619722801000},"page":"569-575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Participating mechanism of a major contributing gene <i>ysnE<\/i> for auxin biosynthesis in <i>Bacillus amyloliquefaciens<\/i> SQR9"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3043-0680","authenticated-orcid":false,"given":"Jiahui","family":"Shao","sequence":"first","affiliation":[{"name":"Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes Nanjing Agricultural University 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