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Nevertheless, the research on the reference genes is still incomplete. Therefore, this study aims to thoroughly investigate the reference genes represented by <jats:italic>Pseudoalteromonas<\/jats:italic> sp. JSTW via whole\u2010genome sequencing. Next\u2010generation sequencing technology unfolded a genome of 4,026,258\u2009bp, database including Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were utilized to annotate the genes and metabolic pathways conferring to petroleum hydrocarbon degradation. The results show that common alkane and aromatic hydrocarbon degradation genes (<jats:italic>alk<\/jats:italic>B, <jats:italic>lig<\/jats:italic>B, <jats:italic>yqh<\/jats:italic>D, and <jats:italic>lad<\/jats:italic>A), chemotaxis gene (MCP, <jats:italic>che<\/jats:italic>A, <jats:italic>che<\/jats:italic>B, <jats:italic>pca<\/jats:italic>Y, and <jats:italic>pca<\/jats:italic>R), heavy\u2010metal resistance, and biofilm genes (\u03c354, <jats:italic>mer<\/jats:italic>C, <jats:italic>pco<\/jats:italic>A, <jats:italic>cop<\/jats:italic>B, etc.) were observed in whole\u2010genome sequence (WGS) of JSTW, which indicated that strain JSTW could potentially cope with combined pollution. The degradation efficiency of naphthalene in 60\u2009h by JSTW was 99% without Cu<jats:sup>2+<\/jats:sup> and 67% with 400\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup> Cu<jats:sup>2+<\/jats:sup>. Comparative genome analysis revealed that genomes of <jats:italic>Pseudoalteromonas lipolytica<\/jats:italic> strain LEMB 39 and <jats:italic>Pseudoalteromonas donghaensis<\/jats:italic> strain HJ51 shared similarity with strain JSTW, suggesting they are also the potential degradater of petroleum hydrocarbons under combined pollution. Therefore, this study provides a WGS annotation and reveals the mechanism of response to combined pollution of <jats:italic>Pseudoalteromonas<\/jats:italic> sp. JSTW.<\/jats:p>","DOI":"10.1002\/jobm.202100156","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T12:04:55Z","timestamp":1628856295000},"page":"947-957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genomic insights into <i>Pseudoalteromonas<\/i> sp. 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