{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T13:03:13Z","timestamp":1793451793506,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Shiraia bambusicola<\/jats:italic> is a fungus with high economic value widely used in medicine, agriculture, and food. We wished\u00a0to understand the genes and metabolites changes involved in the different developmental stages of <jats:italic>S. bambusicola<\/jats:italic>. So, to reveal key genes and metabolites in the main active metabolite,\u00a0the \u00a0were analyzed in different developmental stages of <jats:italic>S. bambusicola<\/jats:italic> fruiting body. A total of 29,137 Unigenes were annotated. In the whole growth process, differentially expressed genes were involved in the pathways of cytochrome P450, transcription factors, transporters, and so on, while in the early stage of growth, genes enriching to synthesis pathways of basic substances. In the middle stage of growth, genes with more prominent changes were involved in the pathways of the cell cycle, cancer mechanisms, and aminobenzoate degradation; in the later stage of growth, differentially expressed genes that enriched synthesis pathways of secondary metabolites. A total of 612 metabolites were detected from different growth stages of <jats:italic>S. bambusicola<\/jats:italic>. Among them, coumarins, alkaloids, rutin, liquiritigenin, quercetin, and other medically relevant metabolites were detected for the first time. We have identified 31 secondary metabolites,\u00a0 relevantly only accumulated in the early and middle stage, but not detected in the later stage, such as flavonols, coumarins, nucleotides and its derivates\u00a0and hydroxycinnamoyl derivatives. The differential genes and metabolites of the same group were enriched in 127 pathways, and more significantly in ubiquinone and other terpenoid quinone biosynthesis, phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and phenylpropanoid biosynthesis. The correlation networks of several significantly enriched pathways were analyzed, and\u00a0the relationships within and between these pathways, genes, and metabolites, were analyzed. The synthetic pathway of hypocrellin has\u00a0been speculated upon. We believe that hypocrellin is synthesized in <jats:italic>S. bambusicola<\/jats:italic> via the shikimic acid pathway followed by phenylalanine, tyrosine, and tryptophan biosynthesis pathway, then the ubiquinone and other terpenoid quinone biosynthesis pathway, and finally a series of polymerization and modification reactions. Several genes and metabolites involved in the biosynthesis of hypocrellin have been identified. This study provides a reference for further research on <jats:italic>S. bambusicola<\/jats:italic>, by providing a basis for its use and development.<\/jats:p>","DOI":"10.1002\/jobm.202200008","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T11:25:54Z","timestamp":1647343554000},"page":"721-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Metabolomics integrated with transcriptomics reveals the changes during developmental stages in <i>Shiraia bambusicola<\/i>"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9692-2306","authenticated-orcid":false,"given":"Xiyi","family":"Ren","sequence":"first","affiliation":[{"name":"College of Agriculture Guizhou University  Guiyang Guizhou China"},{"name":"Institute of Biotechnology Guizhou Academy of Agricultural Sciences  Guiyang Guizhou China"},{"name":"Guizhou Key Laboratory of Agricultural Biotechnology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Tian","sequence":"additional","affiliation":[{"name":"Animal Epidemic Disease Prevention and Control Monitoring Station of Zunyi  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luo","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Guizhou Academy of Agricultural Sciences  Guiyang Guizhou China"},{"name":"Guizhou Key Laboratory of Agricultural Biotechnology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Guizhou Academy of Agricultural Sciences  Guiyang Guizhou China"},{"name":"Guizhou Key Laboratory of Agricultural Biotechnology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Guizhou Academy of Agricultural Sciences  Guiyang Guizhou China"},{"name":"Guizhou Key Laboratory of Agricultural Biotechnology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanli","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Agriculture Guizhou University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Guizhou Academy of Agricultural Sciences  Guiyang Guizhou China"},{"name":"Guizhou Key Laboratory of Agricultural Biotechnology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,15]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"279","article-title":"Studies on systematics, biology and bioactive substance of Shiraia bambusicola","volume":"22","author":"Li XM","year":"2009","journal-title":"Forest Res Beijing"},{"key":"e_1_2_9_3_1","first-page":"952","article-title":"Research and application progress of Shiraia bambusicola","volume":"448","author":"Chen YM","year":"2014","journal-title":"Appl Mech Mater"},{"key":"e_1_2_9_4_1","first-page":"2162","article-title":"Research progresson biological characteristics and medicinal value of fungus Shiraia bambusicola. 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