{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:51:25Z","timestamp":1789167085724,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dengue is an acute arboviral infection common in tropical and subtropical countries. Dengue has been highlighted as a public health concern in the last five decades, affecting almost 50% of the population in developing nations. Dengue infection results in a complex symptomatic disease that ranges from headache, fever, and skin rash to extreme hemorrhage fever and liver dysfunction. The diagnosis of the disease is essential for effective treatment. The early onset of the infection can be detected through viral structural peptides that act as markers for detection, including Pre\u2010Membrane (Pre\u2010M) protein. In the currently proposed research, the structural gene obtained from local isolates was targeted for studies. For this purpose, recombinant structural protein Pre\u2010M was amplified, cloned, and expressed in the bacterial expression system. The expression of the structural protein (Pre\u2010M) was scrutinized by Sodium Dodecyl Sulphate\u2010Polyacrylamide Gel Electrophoresis (SDS\u2010PAGE) and validated by western blot and dot blot, and afterwards, the antigen was purified. The purified Pre\u2010M protein carries the potential for the development of in\u2010house diagnostic assay as well as for vaccine production. This study aimed to develop a highly specific, sensitive, and cost\u2010effective in\u2010house enzyme\u2010linked immunoassay (ELISA) for the detection of antibodies of Pakistani most prevalent dengue virus serotype 2 (DENV\u20102). The success of this research would also pave the way toward developing novel vaccines for the future prevention of dengue infection.<\/jats:p>","DOI":"10.1002\/jobm.202200469","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T20:30:00Z","timestamp":1668112200000},"page":"489-498","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular characterization of recombinant premembrane protein of dengue virus serotype\u20102 for development of diagnostic assay"],"prefix":"10.1002","volume":"63","author":[{"given":"Rakhtasha","family":"Munir","sequence":"first","affiliation":[{"name":"Centre of Applied Molecular Biology (CAMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0575-8246","authenticated-orcid":false,"given":"Shazia","family":"Rafique","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5857-0653","authenticated-orcid":false,"given":"Amjad","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering Hazara University Mansehra Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iram","family":"Amin","sequence":"additional","affiliation":[{"name":"Centre of Applied Molecular Biology (CAMB) University of the Punjab Lahore Pakistan"},{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameen","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Vajeeha","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shahid","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahir R.","family":"Samiullah","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Idrees","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology (CEMB) University of the Punjab Lahore Pakistan"},{"name":"Centre of Biotechnology &amp; Microbiology University of Peshawar Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhi J.","family":"Tao","sequence":"additional","affiliation":[{"name":"Deparment of Biosciences Rice University Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad U.","family":"Khan","sequence":"additional","affiliation":[{"name":"University Institute of Medical lab Technology, Faculty of Allied Health Sciences University of Lahore Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-110615-042334"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes12020231"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccmp.2022.100024"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)00026-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0476-8"},{"key":"e_1_2_9_7_1","article-title":"Overlapping clinical manifestations of COVID\u201019 with endemic infectious diseases in Pakistan: a looming threat of multiple lethal combinations","volume":"11","author":"Rana MS","year":"2021","journal-title":"Infect Ecol Epidemiol"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S347571"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-65625-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.05.088"},{"key":"e_1_2_9_11_1","first-page":"217","article-title":"Genomics, proteomics and evolution of dengue virus","volume":"16","author":"Dwivedi VD","year":"2017","journal-title":"Briefings Funct Genomics"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.17582\/journal.pjz\/2019.51.5.sc4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.193300"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.12.015"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2161"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.5772\/67680"},{"key":"e_1_2_9_17_1","first-page":"390","article-title":"Evaluation of two ELISA assay kits against RT\u2010PCR for diagnosis of dengue virus infection in a hospital setting in Karachi, Pakistan","volume":"59","author":"Khan E","year":"2009","journal-title":"J Pak Med Assoc"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12080829"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2020.1826798"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.05.010"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-009-0426-5"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01919"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0264643"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.202200469","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jobm.202200469","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.202200469","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T02:50:54Z","timestamp":1692586254000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.202200469"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,10]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/jobm.202200469"],"URL":"https:\/\/doi.org\/10.1002\/jobm.202200469","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,10]]},"assertion":[{"value":"2022-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}