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The objective of this study was to characterize phage VP4 (vB_vcM_Kuja), an environmental vibriophage isolated from River Kuja in Migori County, Kenya in 2015. The phenotypic characteristics of the phage were determined using a one\u2010step growth curve, restriction digestion profile, pH, and temperature stability tests. The results revealed that the phage is stable through a wide range of temperatures (20\u221250\u00b0C) and maintains its plaque\u2010forming ability at pH ranging from 6 to 12. The one\u2010step growth curve showed a latent period falling between 40 and 60\u2009min, while burst size ranged from 23 to 30 plaque\u2010forming units\/10\u2009\u00b5l at the same host strain. The restriction digestion pattern using <jats:italic>EcoRI<\/jats:italic>, <jats:italic>SalI<\/jats:italic>, <jats:italic>HindIII<\/jats:italic>, and <jats:italic>XhoI<\/jats:italic> enzymes showed that <jats:italic>HindIII<\/jats:italic> could cut the phage genome. The phage DNA could not be restricted by the other three enzymes. The findings of this study can be used in future studies to determine phage\u2212host interactions.<\/jats:p>","DOI":"10.1002\/jobm.202200635","type":"journal-article","created":{"date-parts":[[2023,1,21]],"date-time":"2023-01-21T03:41:55Z","timestamp":1674272515000},"page":"481-488","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Phenotypic characterization of phage vB_vcM_Kuja"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9425-8955","authenticated-orcid":false,"given":"Alice N.","family":"Maina","sequence":"first","affiliation":[{"name":"Department of Biology University of Nairobi  Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis B.","family":"Mwaura","sequence":"additional","affiliation":[{"name":"Department of Biology University of Nairobi  Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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