{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:47:01Z","timestamp":1786978021137,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:00:00Z","timestamp":1707696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to isolate biosurfactant\u2010producing and hydrocarbon\u2010degrading actinomycetes from different soils using glycerol\u2013asparagine and starch\u2013casein media with an antifungal agent. The glycerol\u2013asparagine agar exhibited the highest number of actinomycetes, with a white, low\u2010opacity medium supporting pigment production and high growth. Biosurfactant analyses, such as drop collapse, oil displacement, emulsification, tributyrin agar test, and surface tension measurement, were conducted. Out of 25 positive isolates, seven could utilize both olive oil and black oil for biosurfactant production, and only isolate RP1 could produce biosurfactant when grown in constrained conditions with black oil as the sole carbon source and inducer, demonstrating in situ bioremediation potential. Isolate RP1 from oil\u2010spilled garden soil is Gram\u2010staining\u2010positive with a distinct earthy odor, melanin formation, and white filamentous colonies. It has a molecular size of ~621\u2009bp and 100% sequence similarity to many\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    spp. Morphological, biochemical, and 16\u2009S\u2009rRNA analysis confirmed it as\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. RP1, showing positive results in all screenings, including high emulsification activity against kerosene (27.2%) and engine oil (95.8%), oil displacement efficiency against crude oil (7.45\u2009cm), and a significant reduction in surface tension (56.7\u2009dynes\/cm).\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. RP1 can utilize citrate as a carbon source, tolerate sodium chloride, resist lysozyme, degrade petroleum hydrocarbons, and produce biosurfactant at 37\u00b0C in a 15\u2009mL medium culture, indicating great potential for bioremediation and various downstream industrial applications with optimization.\n                  <\/jats:p>","DOI":"10.1002\/jobm.202300585","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T15:46:05Z","timestamp":1707752765000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Screening biosurfactant\u2010producing actinomycetes: Identification of\n                    <i>Streptomyces<\/i>\n                    sp. RP1 as a potent species for bioremediation"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4350-4885","authenticated-orcid":false,"given":"Adilah","family":"Ayoib","sequence":"first","affiliation":[{"name":"Faculty of Chemical Engineering &amp; Technology Universiti Malaysia Perlis (UniMAP) Padang Besar Perlis Malaysia"},{"name":"Institute of Nano Electronic Engineering Universiti Malaysia Perlis (UniMAP) Kangar Perlis Malaysia"},{"name":"Carbon Sustainability Nexus (CaSNex), Special Interest Group (SIG) Universiti Malaysia Perlis (UniMAP) Arau Perlis Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-4687","authenticated-orcid":false,"given":"Subash C. B.","family":"Gopinath","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Engineering &amp; Technology Universiti Malaysia Perlis (UniMAP) Padang Besar Perlis Malaysia"},{"name":"Institute of Nano Electronic Engineering Universiti Malaysia Perlis (UniMAP) Kangar Perlis Malaysia"},{"name":"Micro System Technology, Centre of Excellence (CoE) Universiti Malaysia Perlis (UniMAP) Arau Perlis Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nor Syafirah","family":"Zambry","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Universiti Sains Malaysia USM Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Ramli Mohd","family":"Yahya","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Universiti Sains Malaysia USM Pulau Pinang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.21307\/pjm-2018-048"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2019.126374"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e10149"},{"key":"e_1_2_10_5_1","first-page":"212","article-title":"Surfactants tailored by the class actinobacteria","volume":"6","author":"K\u00fcgler JH","year":"2015","journal-title":"Front Microbiol"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-38453-1_7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01664-13"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3897\/folmed.63.e54386"},{"key":"e_1_2_10_9_1","first-page":"293","article-title":"Bioemulsifier production by streptomyces sp. 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