{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:43:36Z","timestamp":1783471416667,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T00:00:00Z","timestamp":1708992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Basic Microbiol"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic>, a multidrug\u2010resistant bacterium has become a significant cause of life\u2010threatening infections acquired in hospitals worldwide. The existing drugs used to treat <jats:italic>A. baumannii<\/jats:italic> infections are rapidly losing efficacy, and the increasing antimicrobial resistance, which is expected to turn into a global health crisis, underscores the urgency to develop novel prevention and treatment strategies. We reasoned that the discovery of novel virulence targets for vaccine and therapy interventions requires a more enhanced method for the introduction of multiple elements of foreign DNA for genome editing than the current methods of natural transformation techniques. Herein, we employed a novel and a much\u2010improved enhanced technique for the natural transformation of elements of the genome editing system CRISPR\u2010Cas9 to suppress specific genomic regions linked to selectively suppress bacterial virulence. We modified the genome of the laboratory\u2010adapted strain of <jats:italic>A. baumannii<\/jats:italic> BAA\u2010747 by targeting the AmpC, as a marker gene, for disruption by three different genomic manipulation strategies, and created mutant strains of <jats:italic>A. baumannii<\/jats:italic> that are, at least, fourfold susceptible to ampicillin. This work has established an optimized enhanced natural transformation system that enables efficient genome editing of pathogenic bacteria in a laboratory setting, providing a valuable future tool for exploring the function of unidentified virulence genes in bacterial genomes.<\/jats:p>","DOI":"10.1002\/jobm.202300644","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T21:09:36Z","timestamp":1709068176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome editing in <i>Acinetobacter baumannii<\/i> through enhanced natural transformation"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5250-3633","authenticated-orcid":false,"given":"Ilknur","family":"Yilmaz","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Graduate School of Science &amp; Engineering Yildiz Technical University Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulin","family":"Ozbek","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Arts and Sciences Yildiz Technical University Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.05.024"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002184"},{"key":"e_1_2_10_5_1","first-page":"21","article-title":"Ulusal sa\u011fl\u0131k hizmeti ili\u015fkili enfeksiyonlar s\u00fcrveyans a\u011f\u0131 (ushiesa) \u00f6zet raporu","volume":"16","author":"Hekimoglu CH","year":"2022","journal-title":"USHIESA"},{"key":"e_1_2_10_6_1","volume-title":"Global priority list of antibiotic\u2010resistant bacteria to guide research, discovery, and development of new antibiotics","author":"Tacconelli E","year":"2021"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020127"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10081006"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24655"},{"key":"e_1_2_10_10_1","first-page":"445","article-title":"Antimicrobial peptides therapy: an emerging alternative for treating drug\u2010resistant bacteria","volume":"95","author":"Mba IE","year":"2022","journal-title":"Yale J Biol Med"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13030352"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201504741"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2147\/BTT.S326422"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.24203"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S370869"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad377"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29877-2"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00754-13"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7020030"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00181-18"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro844"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00951-20"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-021-01242-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.08.058"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v2.i12.787"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pi.6170"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02165"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0246918"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9118-1_8"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00034-21"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2019.09.003"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a012591"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.201500011"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2021.106619"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.19700"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics8010018"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001514"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.096487"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11111239"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2008.1"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.07.022"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.260000"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178700"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906355107"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00030-16"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2015.37"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25053"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000110"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12028-5"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2018.12.002"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-018-0024-7"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030862"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.202300644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T08:09:01Z","timestamp":1717488541000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.202300644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,27]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1002\/jobm.202300644"],"URL":"https:\/\/doi.org\/10.1002\/jobm.202300644","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,27]]},"assertion":[{"value":"2023-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300644"}}