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A novel Gram\u2010positive moderately thermophilic bacterial strain, namely <jats:italic>Bacillus tequilensis<\/jats:italic> ASFS.1, was isolated and identified. This strain is capable of producing superparamagnetic Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> nanoparticles and exhibiting magnetotaxis behavior. This strain swimming behavior was investigated under static and dynamic environments, where it behaved very much similar to the magnetotaxis in magnetotactic bacteria. This study is the first report of a bacterium from the <jats:italic>Bacillaceae<\/jats:italic> family that has the potential to intracellular biosynthesis of IONPs. MNPs were separated by a magnetic and reproducible method which was designed for the first time for this study. In addition, UV\u2010visible spectrophotometer, Fourier\u2010transform infrared spectroscopy, vibrating sample magnetometer, field emission scanning electron microscopy (FESEM), X\u2010ray diffraction, and thermal gravimetric analysis were utilized to characterize the bio\u2010fabricated magnetite nanoparticles. Analysis of the particle size distribution pattern of the biogenic MNPs by FESEM imaging revealed the size range of 10\u2013100\u2009nm with the size range of 10\u201340\u2009nm MNPs being the most frequent particles. VSM analysis demonstrated that biogenic MNPs displayed superparamagnetic properties with a high saturation magnetization value of 184\u2009emu\/g. After 24\u2009h treatment of 3T3, U87, A549, MCF\u20107, and HT\u201029 cell lines with the biogenic MNPs, IC<jats:sub>50<\/jats:sub> values were measured to be 339, 641, 582, 149, and 184\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>, respectively. This study presents the novel strain ASFS.1 capable of magnetotaxis by the aid of its magnetite nanoparticles and paving information on isolation, characterization, and in vitro cytotoxicity of its MNPs. The MNPs showed promising potential for biomedical applications, obviously subject to additional studies.<\/jats:p>","DOI":"10.1002\/jobm.202400153","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T05:55:25Z","timestamp":1719208525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Purification, Characterization, and Assessment of Anticancer Activity of Iron Oxide Nanoparticles Biosynthesized by Novel Thermophilic <i>Bacillus tequilensis<\/i> ASFS1\u200f"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7500-0924","authenticated-orcid":false,"given":"Naghmeh","family":"Satarzadeh","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biotechnology, Faculty of Pharmacy Kerman University of Medical Sciences Kerman 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