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Investigation of <jats:italic>Acanthamoeba<\/jats:italic>\u2013pathogenic bacteria interaction is important for the discovery of new antibacterial agents that can be used against pathogenic bacteria. In this study, it was aimed to investigate the antibacterial effect of cell\u2010free supernatants obtained from <jats:italic>Acanthamoeba<\/jats:italic> against some pathogenic bacteria. One standard strain (<jats:italic>Acanthamoeba castellanii<\/jats:italic> ATCC 50373) and one environmental strain (B1) of the genus <jats:italic>Acanthamoeba<\/jats:italic> were used in the study. Cell\u2010free supernatants were obtained by centrifuging the axenic cultures (3000\u2009rpm, 5\u2009min) and passing through a sterile filter with a pore diameter of 0.22\u2009\u00b5m. The antibacterial effect of cell\u2010free supernatants against five different pathogenic bacteria (<jats:italic>Acinetobacter baumannii<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Enterococcus faecalis<\/jats:italic>, <jats:italic>Salmonella<\/jats:italic> Typhi, <jats:italic>and Salmonella enterica<\/jats:italic>) was investigated by colony counting method. As a result of the study, it was determined that the standard <jats:italic>Acanthamoeba<\/jats:italic> cell\u2010free supernatant showed the highest antibacterial effect against <jats:italic>E. faecalis<\/jats:italic> (75.79%), while B1 cell\u2010free supernatant showed the highest antibacterial effect against <jats:italic>K. pneumoniae<\/jats:italic> (8.5%). The content of the tested <jats:italic>Acanthamoeba<\/jats:italic> cell\u2010free supernatants was analyzed by gas chromatography\/mass spectrometry in our previous study and was also found to contain major compounds with antibacterial properties. Therefore, it is thought that the metabolites produced by <jats:italic>Acanthamoeba<\/jats:italic> can be used as an alternative to existing antimicrobial drugs in the fight against infections caused by some important pathogenic bacteria.<\/jats:p>","DOI":"10.1002\/jobm.202400537","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T05:29:17Z","timestamp":1734067757000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of <i>Acanthamoeba<\/i> Spp. Cell\u2010Free Supernatants on Some Bacterial Pathogens"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3336-5618","authenticated-orcid":false,"given":"\u015eevval Maral","family":"\u00d6zcan Aykol","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology Faculty of Pharmacy, Biruni University Istanbul Turkey"},{"name":"Biruni University Research Center (B@MER) Biruni 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