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The present study was conducted to formulate an efficient and stable liquid bacterial inoculant (LBI) of <jats:italic>Acinetobacter lwoffii<\/jats:italic> strain PAU_31LN. In the current investigation, total 16 endophytic bacteria were isolated from cotton leaves and evaluated for plant growth\u2010promoting features such as production of phytohormones, mineral solubilization, siderophore production, hydrogen cyanide (HCN) production, and 1\u2010aminocyclopropane\u20101\u2010carboxylate (ACC) deaminase activity. The leaf endophytic bacteria designated as 31LN was found promising for all the PGP traits and it was identified as <jats:italic>A. lwoffii<\/jats:italic> strain PAU_31LN by 16S rRNA gene sequencing. For the development of LBI of <jats:italic>A. lwoffii<\/jats:italic> strain PAU_31LN, 4.5\u2009g\/L yeast extract, 5\u2009g\/L NaCl, 5\u2009g\/L peptone, and 12.5\u2009mM food\u2010grade trehalose was optimized as appropriate medium composition using response surface methodology (RSM) and Box\u2013Behnken design. Further, the viability of <jats:italic>A. lwoffii<\/jats:italic> strain PAU_31LN in the optimized formulation was observed as 1.1 folds higher over the control after 180 days of storage at room temperature. Moreover, nonsignificant variation was recorded in the functional traits of 180 days old LBI of <jats:italic>A. lwoffii<\/jats:italic> strain PAU_31LN and freshly prepared LBI. The in\u2010vitro plant growth parameters such as length and seed vigor index of 7\u2010day\u2010old cotton seedlings were enhanced by the seed bio\u2010priming with LBI of <jats:italic>A. lwoffii<\/jats:italic> strain PAU_31LN over the control. The results of the present study signify the importance of endophytes and statistical methods to formulate prominent LBI.<\/jats:p>","DOI":"10.1002\/jobm.202400617","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T05:45:51Z","timestamp":1737351951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development, Shelf Stability, and In\u2010Vitro Evaluation of Liquid Bacterial Inoculant <i>Acinetobacter lwoffii<\/i> Strain PAU_31LN"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9850-501X","authenticated-orcid":false,"given":"Jagjot","family":"Kaur","sequence":"first","affiliation":[{"name":"Department of Microbiology Punjab Agricultural University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulab","family":"Pandove","sequence":"additional","affiliation":[{"name":"School of Organic Farming Punjab Agricultural University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vineet","family":"Kumar","sequence":"additional","affiliation":[{"name":"Regional Research Station, Bathinda Punjab Agricultural University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal Abdullah A.","family":"Sabour","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"Alshiekheid","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00745"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-022-04052-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.15796"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/su13052856"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/su13031140"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061551"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.953836"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fsufs.2022.900230"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/agriculture12040462"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13237"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-02-17-0051-R"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-024-02397-w"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.12972\/kjhst.20170002"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.781764"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26195758"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-024-02435-7"},{"key":"e_1_2_10_18_1","first-page":"125","volume-title":"Phyllosphere Microbiology","author":"Holland M. 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