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This natural tolerance was used for counter\u2010selection of <jats:italic>trans<\/jats:italic>\u2010conjugants in heterospecific matings with <jats:italic>Escherichia coli<\/jats:italic> as donor. This approach is useful for gentic manipulations in <jats:italic>Legionella<\/jats:italic>, as it avoids the use of antibiotic\u2010resistant variants, which have to be tested for full virulence before use.<\/jats:p>","DOI":"10.1002\/jobm.3620320616","type":"journal-article","created":{"date-parts":[[2007,2,5]],"date-time":"2007-02-05T21:28:26Z","timestamp":1170710906000},"page":"423-426","source":"Crossref","is-referenced-by-count":4,"title":["Ampicillin tolerance of <i>Legionella pneumophila<\/i> for counter\u2010selection of transconjugants in heterospecific matings with <i>Escherichia coli<\/i> donors"],"prefix":"10.1002","volume":"32","author":[{"given":"Bunty","family":"Jain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larisa","family":"Bender","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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