{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:58:26Z","timestamp":1753869506543,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T00:00:00Z","timestamp":1742688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Esteya vermicola<\/jats:italic> Fxy121 strain, isolated from live pine trees, exhibited high adhesion and infection activity against <jats:italic>Bursaphelenchus xylophilus<\/jats:italic>. In this study, we studied the biological characteristics of <jats:italic>Esteya vermicola<\/jats:italic> Fxy121, and screened species\u2010specific primers used for its colonization detection on pine trees. The liquid fermentation formula with higher blastospore production was obtained by adjusting the ratio of carbon and nitrogen and adding mineral salts, blastospore concentration was 5.5\u2009\u00d7\u200910<jats:sup>8<\/jats:sup>\u2009spores\u2009mL<jats:sup>\u22121<\/jats:sup> cultured for 6 days using new formula. The germination of blastospores was not affected when suspension was placed at 4\u00b0C\u201330\u00b0C for 1\u20138\u2009h. The number of lunate spores increased first and then decreased with the increase of initial blastospore concentration of <jats:italic>E. vermicola<\/jats:italic> Fxy121, medium concentration of blastospores suspension showed higher infectivity. The evF\/evR primer pair successfully amplified the except fragment size of 522\u2009bp from isolates of <jats:italic>E. vermicola<\/jats:italic> and from pine needles sprayed with blastospores suspension of <jats:italic>E. vermicola<\/jats:italic>. This study suggests that blastospores concentration of <jats:italic>E. vermicola<\/jats:italic> Fxy121 significantly affect the production of lunate spores and infectivity against <jats:italic>B. xylophilus<\/jats:italic>. The species\u2010specific primers allowed fast and reliable detection of <jats:italic>E. vermicola<\/jats:italic> directly from tissues of pine trees sprayed and injected with spore suspension.<\/jats:p>","DOI":"10.1002\/jobm.70019","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T07:10:28Z","timestamp":1742800228000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Biological Characteristics of Nematophagous Fungus <i>Esteya vermicola<\/i> Fxy121 Strain and Its Specific Detection"],"prefix":"10.1002","volume":"65","author":[{"given":"Xiaojian","family":"Wen","sequence":"first","affiliation":[{"name":"Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China"},{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxia","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China"},{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangtong","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China"},{"name":"Beijing Key Laboratory for Forest Pests Control, College of Forestry Beijing Forestry University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China"},{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China"},{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Huang","sequence":"additional","affiliation":[{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China"},{"name":"Co\u2010Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-75655-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-011-9924-x"},{"key":"e_1_2_9_4_1","unstructured":"National Forestry and Grassland Administration Bulletin of the National Forestry and Grassland Administration (No. 4 2024). 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