{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T20:06:08Z","timestamp":1767211568650,"version":"3.43.0"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T00:00:00Z","timestamp":1746921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Multidrug\u2010resistant (MDR) Gram\u2010negative bacteria constitute a major global health concern, and the increasing resistance to last\u2010line antimicrobial agents compels the urgent improvement of novel treatment strategies. The present study aims to produce colistin and ceftazidime\u2013avibactam conjugated silver nanoparticles by eco\u2010friendly synthesis method and to evaluate the antimicrobial and antibiofilm activities against MDR Gram\u2010negative bacteria. The physicochemical characteristics of nanoparticles were analyzed through ultraviolet\u2010visible spectrophotometry, high resolution\u2010transmission electron microscopy, X\u2010ray diffraction, and Fourier transform infrared spectroscopy. Broth microdilution assay was conducted to detect minimum inhibitory concentration (MIC) levels of nanoparticles against MDR bacteria and reference strains. The antibiofilm efficacy was assessed by the crystal violet staining method, and the cytotoxic activity was determined using the MTT assay. The characterization analyses demonstrated spherical morphology (5.6\u201341.6\u2009nm in diameter) and crystalline structures for the nanoparticles. The MIC levels of antibiotic\u2010conjugated nanoparticles against reference strains and MDR clinical isolates ranged from 0.125 to 4\u2009\u00b5g\/mL, showing inhibitory concentrations (2\u20131024 fold) lower than that of free antibiotics (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Nanoparticles exhibited more effective biofilm activity on <jats:italic>Pseudomonas aeruginosa<\/jats:italic> PAO1 strain compared to free antibiotics, and showed no substantial inhibitory impact on cell viability, even at increased concentrations. The nanoparticles obtained by the green synthesis method could be promising agents in fighting against MDR Gram\u2010negative bacteria.<\/jats:p>","DOI":"10.1002\/jobm.70044","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T01:19:09Z","timestamp":1747012749000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antibacterial and Antibiofilm Activities of Colistin and Ceftazidime\u2013Avibactam Conjugated Silver Nanoparticles"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6433-3726","authenticated-orcid":false,"given":"Yasemin","family":"Cezaro\u011flu","sequence":"first","affiliation":[{"name":"Medical Microbiology Laboratory Unye State Hospital Ordu Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7349-3438","authenticated-orcid":false,"given":"M\u00fcmtaz Cem","family":"\u015eirin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Suleyman Demirel University Isparta Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di\u011fdem","family":"Trak","sequence":"additional","affiliation":[{"name":"Department of Nanoscience and Nanotechnology, Faculty of Science and Literature Burdur Mehmet Akif Ersoy University Burdur Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emel Sesli","family":"\u00c7etin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Suleyman Demirel University Isparta Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasin","family":"Arslan","sequence":"additional","affiliation":[{"name":"Department of Nanoscience and Nanotechnology, Faculty of Science and Literature Burdur Mehmet Akif Ersoy University Burdur Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buket","family":"Ar\u0131do\u011fan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Suleyman Demirel University Isparta Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,11]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"1","article-title":"Common Problems and Solutions in the Management of Resistant Gram\u2010Negative Bacterial Infections: Expert Opinion on Clinical Practice","volume":"29","author":"S\u00fcmer \u015e.","year":"2024","journal-title":"Flora"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2015.993381"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2019.03.009"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics7020050"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S121956"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2015.02.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02980"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2589"},{"key":"e_1_2_10_10_1","first-page":"1","article-title":"Essential Oils and Phenolic Compounds, Antiradical and Antioxidant Activities of Distillation Products in Oil\u2010Bearing Rose (Rosa damascena Mill.)","volume":"23","author":"Baydar H.","year":"2017","journal-title":"Journal of Agricultural Science"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b22043"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.09.045"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.202300440"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S198194"},{"key":"e_1_2_10_15_1","first-page":"1","article-title":"Synthesis of Silver Nanoparticles Using Dried Black Mulberry (Morus nigra L.) 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