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These microorganisms demonstrate unique mechanisms of action that focus on specific biological targets. For example, bacteria such as\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    focus on the synthesis of the cell wall, while\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    targets the replication of DNA. Fungi like\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    interfere with ergosterol synthesis, while\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    targets lanosterol 14\u03b1\u2010demethylase. Viruses like HIV focus on reverse transcriptase, whereas the\n                    <jats:italic>Influenza<\/jats:italic>\n                    virus targets neuraminidase. Parasites such as\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    focus on dihydrofolate reductase, and\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    targets dihydropteroate synthase. Understanding these biological targets is crucial for creating effective treatment strategies against infectious diseases. Drugs are essential for influencing particular biological functions in microorganisms, which in turn hampers their growth, persistence, or reproduction. Antibacterial drugs, including beta\u2010lactams and fluoroquinolones, focus on disrupting cell wall formation and DNA replication in bacteria, respectively. Antifungal drugs, including azoles and polyenes, focus on inhibiting ergosterol production and maintaining cell membrane stability in fungi. Antiviral medications, such as reverse transcriptase inhibitors and protease inhibitors, aim at interfering with viral replication and maturation processes in viruses. Antiparasitic medications, like antifolates and arylaminoalcohols, target dihydrofolate reductase and various crucial enzymes in parasites. By targeting these biological processes, drugs can effectively treat infectious diseases caused by microorganisms. This review will address the understanding of microorganisms biological targets for antimicrobial medications by their mechanisms of action.\n                  <\/jats:p>","DOI":"10.1002\/jobm.70057","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T04:26:11Z","timestamp":1747628771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Comprehensive Review of Antimicrobial Drugs: Mechanisms of Action and Specific Targets in Microorganisms"],"prefix":"10.1002","volume":"65","author":[{"given":"Deepika","family":"Shekhawat","sequence":"first","affiliation":[{"name":"Department of Biotechnology REVA University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0077-6995","authenticated-orcid":false,"given":"Kuruvalli","family":"Gouthami","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University Bangalore Karnataka India"},{"name":"DR Biosciences, Research and Development Unit, Bettahalasuru Bangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayantika","family":"Santra","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhasish","family":"Maity","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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