{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T23:01:07Z","timestamp":1772665267650,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Hyperendemic zoonotic sporotrichosis, attributed to <jats:italic>Sporothrix brasiliensis<\/jats:italic>, presents a significant public health challenge in Brazil. Cats exhibit severe symptoms and high fungal loads, though their susceptibility is unclear. <jats:italic>Sporothrix schenckii<\/jats:italic> can also cause feline disease, primarily seen in Asia. This study is the first to report an in vitro model for examining cat immune cell responses to <jats:italic>S. brasiliensis<\/jats:italic> or <jats:italic>S. schenckii<\/jats:italic>. We investigated the phagocytic activity of blood cells (FMdP) from healthy domestic cats, challenged with yeast cells of <jats:italic>S. brasiliensis<\/jats:italic> and <jats:italic>S. schenckii<\/jats:italic>. The survival of these yeasts within cat phagocytes and their cytotoxic effect on host cells were monitored. Both fungal species developed and replicated within feline phagocytes while <jats:italic>S. brasiliensis<\/jats:italic> phagocytic index (PI) was higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Interspecies analyses showed that <jats:italic>S. schenckii<\/jats:italic> required a higher multiplicity of infection to be more cytotoxic than <jats:italic>S. brasiliensis<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009\u2264\u20090.01). The present report brings relevant information to understand <jats:italic>S. brasiliensis<\/jats:italic> host adaptation and, ultimately, cat susceptibility to sporotrichosis. This pioneering study on the feline's innate immune response provides new insights for future complex studies such as those involving fungal ligand recognition by cat cell receptors.<\/jats:p>","DOI":"10.1002\/jobm.70077","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T05:39:33Z","timestamp":1752557973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Novel In Vitro Host\u2212Pathogen Model for <i>Felis catus<\/i> and <i>Sporothrix<\/i> Zoonotic Species Investigation"],"prefix":"10.1002","volume":"65","author":[{"given":"Gabriele Barros","family":"Moth\u00e9","sequence":"first","affiliation":[{"name":"Center for Microorganisms' Investigation, Biomedical Institute Federal Fluminense University Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nath\u00e1lia 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