{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T02:30:11Z","timestamp":1776738611393,"version":"3.51.2"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The putative two\u2010component system (TCS) CgtRS5, encoded by the <jats:italic>ncgl2572<\/jats:italic>\u2013<jats:italic>ncgl2573<\/jats:italic> gene cluster, plays a critical role in environmental adaptation in <jats:italic>Corynebacterium glutamicum<\/jats:italic>. Comparative transcriptomic analysis between the wild\u2010type strain and a \u0394<jats:italic>cgtRS5<\/jats:italic> mutant revealed three upregulated and 107 downregulated genes in the mutant. Among these, genes involved in iron metabolism (<jats:italic>ncgl1959<\/jats:italic>), aromatic compound degradation (<jats:italic>ncgl2320<\/jats:italic>), and drug resistance (<jats:italic>cssR<\/jats:italic>) were significantly downregulated. Phenotypic assays demonstrated that the \u0394<jats:italic>cgtRS5<\/jats:italic> mutant exhibited impaired growth in media supplemented with the divalent form of iron (Fe\u00b2\u207a) or the alkylating agent iodoacetamide (IAM), but showed no significant differences under benzoate, resorcinol, or antibiotic stress. These results suggest that CgtRS5 specifically regulates the stress response to iron and IAM in <jats:italic>C. glutamicum<\/jats:italic>, providing new insights into TCS\u2010mediated regulatory mechanisms in this organism.<\/jats:p>","DOI":"10.1002\/jobm.70085","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T06:19:46Z","timestamp":1753683586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Function and Regulatory Mechanism of a Two\u2010Component Signal Transduction System <i>cgtRS5<\/i> in <i>Corynebacterium glutamicum<\/i>"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2855-8318","authenticated-orcid":false,"given":"Zhaoxin","family":"Peng","sequence":"first","affiliation":[{"name":"College of Life Sciences Qufu Normal University Qufu 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