{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T20:41:01Z","timestamp":1769719261553,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    is considered a major extended\u2010spectrum \u03b2\u2010lactamases producer, of which CTX\u2010M is the most common member. However, the different variants of this primary resistance\u2010determinant make it challenging to detect by conventional PCR\u2010based techniques. Therefore, in this study, we investigated the transcriptional response of 2 secondary resistance genes,\n                    <jats:italic>repB<\/jats:italic>\n                    and\n                    <jats:italic>mtgA<\/jats:italic>\n                    , under sub\u2010inhibitory concentrations of oxy\u2010imino cephalosporins in resistant isolates of\n                    <jats:italic>E. coli<\/jats:italic>\n                    harboring\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    . The STRING database and Cytoscape software were used to identify the secondary resistance genes by gene network analysis. The cephalosporin\u2010resistant isolate of\n                    <jats:italic>E. coli<\/jats:italic>\n                    co\u2010harboring\n                    <jats:italic>repB<\/jats:italic>\n                    and\n                    <jats:italic>mtgA<\/jats:italic>\n                    , along with\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    , was employed for the current study. The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    \u2010carrying plasmid was successfully eliminated from the native\u2010type isolate using 6% and 8% SDS treatment for 7 days. The plasmid was also transformed into a recipient strain,\n                    <jats:italic>E. coli<\/jats:italic>\n                    DH5\u03b1, by the heat\u2010shock method. All three models were grown under sub\u2010inhibitory stress of cephalosporins, and the transcriptional response of\n                    <jats:italic>repB<\/jats:italic>\n                    and\n                    <jats:italic>mtgA<\/jats:italic>\n                    was determined by quantitative real\u2010time PCR. The study revealed that the transcriptional responses of both\n                    <jats:italic>repB<\/jats:italic>\n                    and\n                    <jats:italic>mtgA<\/jats:italic>\n                    were antibiotic\u2010specific, which potentially can serve as a secondary resistance reporter for cephalosporin resistance in\n                    <jats:italic>E. coli<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/jobm.70135","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T09:56:34Z","timestamp":1767002194000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oxy\u2010Imino\u2010Cephalosporin Induces Expression of Secondary Resistance Genes\n                    <i>repB<\/i>\n                    and\n                    <i>mtgA<\/i>\n                    in\n                    <i>Escherichia coli<\/i>\n                    Harboring\n                    <i>bla<\/i>\n                    <sub>CTX\u2010M<\/sub>\n                    ESBL"],"prefix":"10.1002","volume":"66","author":[{"given":"Nikita","family":"Karmakar","sequence":"first","affiliation":[{"name":"Department of Microbiology Assam University  Silchar Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayalaxmi","family":"Wangkheimayum","sequence":"additional","affiliation":[{"name":"Department of Microbiology Assam University  Silchar Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debadatta Dhar","family":"Chanda","sequence":"additional","affiliation":[{"name":"Department of Microbiology Silchar Medical College and Hospital  Silchar Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabendra Dutta","family":"Choudhury","sequence":"additional","affiliation":[{"name":"Rabindranath Tagore University  Hojai Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3111-9835","authenticated-orcid":false,"given":"Amitabha","family":"Bhattacharjee","sequence":"additional","affiliation":[{"name":"Department of Microbiology Assam University  Silchar Assam India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16111615"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.07.002"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10010069"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.18203\/2394-6040.ijcmph20173306"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_331_18"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/picr.PICR_29_20"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.657-686.2005"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2012.27.2.128"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.115918"},{"key":"e_1_2_11_12_1","article-title":"CTX\u2010M\u2010Type 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