{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:34:53Z","timestamp":1772998493681,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study aimed to isolate and evaluate actinobacterial isolates for their antifungal potential against phytopathogenic fungi. A total of 20 morphologically distinct actinobacterial isolates were obtained from soil samples and screened against\n                    <jats:italic>Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus<\/jats:italic>\n                    , and\n                    <jats:italic>Penicillium<\/jats:italic>\n                    sp. Among these, isolate RS18 exhibited the highest antifungal activity and was identified as\n                    <jats:italic>Streptomyces levis<\/jats:italic>\n                    based on 16S rRNA gene sequencing. Cultural characterization of this potent strain on various International Streptomyces Project media revealed distinct colony morphologies and pigment production patterns. Response Surface Methodology was employed to optimize the production conditions, and the ethyl acetate extract from the optimized culture demonstrated strong antifungal activity in both well diffusion and minimum fungicidal concentration assays. GC\u2010MS analysis of the crude extract identified multiple bioactive compounds, including esters, alcohols, and nitrogen\u2010containing heterocycles. ADMET analysis of the top compounds indicated high gastrointestinal absorption, non\u2010mutagenicity, good drug\u2010likeness, and predicted hepatotoxicity. Molecular docking studies revealed that 3,8\u2010dimethyl\u20103\u2010(4\u2010methylpent\u20103\u2010enyl)\u201011H\u2010pyrano[3,2\u2010a] carbazole had the highest binding affinity (\u20135.53\u2009kcal\/mol) with the Aspergillus hydrolase protein (1UKC), followed by other compounds with moderate binding energies. These results suggest that the strain\n                    <jats:italic>S. levis<\/jats:italic>\n                    RS18 is a promising biocontrol candidate against fungal phytopathogens and may be beneficial in agricultural practices to enhance crop production in target organisms.\n                  <\/jats:p>","DOI":"10.1002\/jobm.70157","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T09:44:54Z","timestamp":1772963094000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["GC\u2013MS Profiling and In Silico Evaluation of\n                    <i>Streptomyces levis<\/i>\n                    Strain RS18 Against Phytopathogenic Fungi"],"prefix":"10.1002","volume":"66","author":[{"given":"Pavithra","family":"Velu","sequence":"first","affiliation":[{"name":"Actinobacterial Research Laboratory, Department of Microbiology Periyar University  Salem Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lokesh","family":"Elumalai","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Helix Research Studio, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nithyalakshmi","family":"Mohanam","sequence":"additional","affiliation":[{"name":"Actinobacterial Research Laboratory, Department of Microbiology Periyar University  Salem Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sivarajan","family":"Anbalmani","sequence":"additional","affiliation":[{"name":"Actinobacterial Research Laboratory, Department of Microbiology Periyar University  Salem Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balagurunathan","family":"Ramasamy","sequence":"additional","affiliation":[{"name":"Actinobacterial Research Laboratory, Department of Microbiology Periyar University  Salem Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-018-0793-y"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.670135"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643-025-00849-w"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819583.ch34"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology12060809"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-021-00625-w"},{"issue":"2020","key":"e_1_2_12_8_1","first-page":"111","article-title":"Effective Role of Beneficial Microbes in Achieving the Sustainable Agriculture and Eco\u2010Friendly Environment Development Goals: A Review","volume":"5","author":"Ahirwar N. 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