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Since humans lack the\n                    <jats:italic>de novo<\/jats:italic>\n                    synthesis of biotin, bacterial biotin synthesis pathway is an attractive target for the development of new antibacterial. The pathway is divided into two phases: the early and late segments. The late stage of biotin biosynthesis is a conserved pathway in which biotin is synthesized from a pimelate precursor using four conserved enzymes, whereas the early stage remained largely unknown until its discovery in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    in 2010. Since then, the early stage of biotin biosynthesis has emerged as divergent and varies among different species. In this review, the early stage of biotin biosynthesis and its diversity across species is discussed. 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