{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T06:57:33Z","timestamp":1790578653322,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Basic Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pycnoporus sanguineus<\/jats:italic>\n                    is a medicinally important macrofungus recognized for its ability to produce diverse bioactive secondary metabolites, including extracellular pigments with potential biotechnological and therapeutic potential. The present study evaluated the growth characteristics, biomass production under solid\u2010state and submerged culture conditions, pigment formation, and bioactivity of\n                    <jats:italic>P. sanguineus<\/jats:italic>\n                    . Among the submerged conditions, agitated cultivation in potato dextrose broth (PDB) produced the highest mycelial biomass (1.16\u2009g dry weight) after 25\u2009days of incubation. Comparative evaluation on six solid media revealed maximum radial growth on potato dextrose agar (PDA) with a growth rate of 74.66\u2009mm within 120\u2009h and a specific growth rate of 0.09048\u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Pigment production was further assessed using modified PDA culture formulations and quantified by a novel ArcGIS\u2010based digital image analysis approach. Pigment from all five formulations was extracted using accelerated solvent extraction (ASE) with ethanol and subjected to UV\u2010Visible spectrophotometric analysis for comparative assessment of relative pigment accumulation. Spectrophotometric analysis at 416\u2009nm showed a positive correlation (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.8005) with the ArcGIS\u2010derived pigmented area, validating image\u2010based assessment of relative pigment accumulation. LC\u2010MS analysis identified pigment\u2010associated metabolites, including cinnabarinic acid, tramesanguin, lambertellin, and coibanoles A and B. The pigment extracts exhibited significant antioxidant and concentration\u2010dependent antibacterial activities against\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    , with MIC values supporting their antibacterial potential. Collectively, the results demonstrated that integrating Arc\u2010GIS\u2010based image analysis, UV\u2010visible spectrophotometric, and LC\u2010MS profiling provides a robust approach for the characterization and biological evaluation of\n                    <jats:italic>P. sanguineus<\/jats:italic>\n                    pigments, highlighting their potential for further application.\n                  <\/jats:p>","DOI":"10.1002\/jobm.70204","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:20:44Z","timestamp":1790245244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Image\u2010Assisted Quantification Using Arc GIS and LC\u2010Ms Based Characterization of Pigments in\n                    <i>Pycnoporus sanguineus<\/i>"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2539-7969","authenticated-orcid":false,"given":"Ithi","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Rural Technology and Social Developament Guru Ghasidas Vishwavidyalaya (A Central University), Koni  Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayank","family":"Pandey","sequence":"additional","affiliation":[{"name":"Technology Enabling Centre Guru Ghasidas Vishwavidyalaya (A Central University), Koni  Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5213-2433","authenticated-orcid":false,"given":"Monika","family":"Bhadauria","sequence":"additional","affiliation":[{"name":"Department of Zoology Guru Ghasidas Vishwavidyalaya (A Central University), Koni  Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8175-5274","authenticated-orcid":false,"given":"Rajendra","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Rural Technology and Social Developament Guru Ghasidas Vishwavidyalaya (A Central University), Koni  Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2991-2906","authenticated-orcid":false,"given":"Bhaskar","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Department of Rural Technology and Social Developament Guru Ghasidas Vishwavidyalaya (A Central University), Koni  Bilaspur Chhattisgarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2023.51227"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2021.1901647"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1890"},{"key":"e_1_2_10_5_1","first-page":"75","article-title":"Anti\u2010Inflammatory Principles of Cultivated Pycnoporus Sanguineus","volume":"21","author":"Lu M. 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