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Here, a defined two\u2010member consortium was assembled, comprising\n                    <jats:italic>Acinetobacter johnsonii<\/jats:italic>\n                    (AJ), a frequently recovered species in wastewater treatment plants, and\n                    <jats:italic>Comamonas testosteroni<\/jats:italic>\n                    (CT); its denitrification behaviour was dissected using time\u2010resolved performance assays, a nitrogen mass balance, 16S rRNA community tracking, and quantitative proteomics. All cultures removed more than 99% of influent NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2013N (initial ~100\u2009mg\u2009N\/L) within 12\u2009h. In monoculture, CT accumulated more N\n                    <jats:sub>2<\/jats:sub>\n                    O (4.11\u2009\u00b5g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    N\n                    <jats:sub>2<\/jats:sub>\n                    O\u2013N) and less residual nitrite, whereas AJ showed the opposite pattern (0.28\u2009\u00b5g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    N\n                    <jats:sub>2<\/jats:sub>\n                    O\u2013N). The 1:1 consortium reconciled both liabilities, limiting nitrite build\u2010up and holding N\n                    <jats:sub>2<\/jats:sub>\n                    O\u2013N to 0.37\u2009\u00b5g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    , approximately one order of magnitude below CT. CT expressed a canonical periplasmic denitrification module (periplasmic nitrate reductase NapAB and norB, a cbb3\u2010type cytochrome\u2010c oxidase related to nitric oxide reductase) but lacked detectable nitrous oxide reductase (NosZ), consistent with partial denitrification and N\n                    <jats:sub>2<\/jats:sub>\n                    O accumulation. AJ instead expressed an assimilatory nitrate\u2010reduction repertoire (nitrate transporter F946_02194 and NAD(P)H\u2010dependent nitrite reductase subunits F946_02190\/02191), consistent with nitrate assimilation into biomass rather than complete denitrification to N\n                    <jats:sub>2<\/jats:sub>\n                    . We propose thcD\u2010derived electron cross\u2010feeding from CT to AJ supports this assimilatory route, consistent with redirecting nitrogen away from gaseous intermediates. The consortium raised intracellular nitrogen to 25.3% of the initial nitrogen pool while minimising N\n                    <jats:sub>2<\/jats:sub>\n                    O, suggesting a community\u2010engineering principle for low\u2010N\n                    <jats:sub>2<\/jats:sub>\n                    O nitrogen removal.\n                  <\/jats:p>","DOI":"10.1002\/jobm.70209","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:33:31Z","timestamp":1792665211000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Synthetic Consortium of Comamonas Testosteroni and Acinetobacter Johnsonii Suppresses Nitrous Oxide Emission"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5487-6857","authenticated-orcid":false,"given":"Juvens Sugira","family":"Murekezi","sequence":"first","affiliation":[{"name":"Institute of Urban Environment, Chinese Academy of 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