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On the 4th postisolation day either [<jats:sup>35<\/jats:sup>S]sulfate or [<jats:sup>3<\/jats:sup>H]thymidine was added to the medium, and the pellets were exposed for 24 h to capacitively coupled electrical fields (10, 100, 250, and 1,000 V peak\u2010to\u2010peak, 60 kHz, sine wave signals). The pellets were then harvested, dialyzed, hydrolyzed, and assayed for DNA, protein, [<jats:sup>35<\/jats:sup>S]sulfate incorporation, and [<jats:sup>3<\/jats:sup>H]thymidine incorporation. Results indicated that at 250 V peak\u2010to\u2010peak there was a statistically significant increase in [<jats:sup>35<\/jats:sup>S]sulfate in 1% NBCS and a statistically significant increase in [<jats:sup>3<\/jats:sup>H]thymidine in 10% NBCS. At potentials above or below 250 V no changes were noted. Thus, articular cartilage chondrocytes grown in pellet form can be stimulated to increase glycosaminoglycan synthesis or to increase cell proliferation by an appropriate capacitively coupled electrical field. The importance of the serum concentration in the medium in evaluation of biosynthesis <jats:italic>in vitro<\/jats:italic> is noted.<\/jats:p>","DOI":"10.1002\/jor.1100020104","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T13:15:04Z","timestamp":1109250904000},"page":"15-22","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["<i>In vitro<\/i> growth of bovine articular cartilage chondrocytes in various capacitively coupled electrical fields"],"prefix":"10.1002","volume":"2","author":[{"given":"Carl T.","family":"Brighton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony S.","family":"Unger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffery L.","family":"Stambough","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90460-5"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(77)90150-2"},{"key":"e_1_2_1_4_2","first-page":"401","volume-title":"Electrical Properties of Bone and Cartilage","author":"Ashihara T","year":"1979"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197407000-00029"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1974.tb26815.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.137.3535.1063"},{"key":"e_1_2_1_8_2","first-page":"427","volume-title":"Electrical Properties of Bone and Cartilage","author":"Bassett CAL","year":"1979"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.178.4064.982"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/204652a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.184.4136.575"},{"key":"e_1_2_1_12_2","first-page":"311","volume-title":"Electrical Properties of Bone and Cartilage","author":"Bassett LS","year":"1979"},{"key":"e_1_2_1_13_2","first-page":"65","volume-title":"Fracture Treatment and Repair","author":"Brighton CT","year":"1980"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"971","DOI":"10.2106\/00004623-197658070-00012","article-title":"In vitro epiphyseal plate growth in various constant electrical fields","volume":"58","author":"Brighton CT","year":"1976","journal-title":"J Bone Joint Surg [Am]"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100010106"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008208009152295"},{"key":"e_1_2_1_17_2","first-page":"199","article-title":"Studies on the regeneration of ossal tissue. 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