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Neutral metalloprotease activity was determined by radiolabeled substrate assays and substrate gel analysis. The enzymes were activated with 4\u2010aminophenylmercuric acetate and were inhibited by 1, 10\u2010phenanthroline and ethylenediamine tetraacetic acid. Protein immunoblots demonstrated that type I collagenase and stromelysin (matrix metalloproteinase III) secretion was increased following staphylococcal medium challenge. The profile of enzymatic activity induced by staphylococcal medium was directly comparable to that observed with interleukin\u20101, which was used as a positive control. The staphylococcal medium had no inherent proteolytic activity. Increased production of the neutral metalloproteases collagenase and stromelysin may significantly contribute to the extensive cartilage destruction noted in staphylococcal septic arthritis.<\/jats:p>","DOI":"10.1002\/jor.1100090214","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T11:55:22Z","timestamp":1109246122000},"page":"258-265","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Purified staphylococcal culture medium stimulates neutral metalloprotease secretion from human articular cartilage"],"prefix":"10.1002","volume":"9","author":[{"suffix":"III","given":"Riley J.","family":"Williams","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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