{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T13:11:41Z","timestamp":1766754701676},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":4858,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[1991,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonaggregated proteoglycan monomers, digested fragments of the monomers, as well as link proteins have been shown to self\u2010associate. These associations have not been shown to occur on the aggregate. However, previous reports, using the Kleinschmidt technique of monolayer electron microscopy, have noted proteoglycan subunits on the aggregate that appear to interact, either as branched proteoglycans or as proteoglycan subunits that appear to share the same attachment site on the hyaluronic acid chain. Branching and shared attachments were noted in all aggregates analyzed in this study. Increasing the average space between proteoglycan subunits on the reconstituted aggregated resulted in a significant decrease in branched proteoglycans, indicating either a weak association occurring on the aggregate, or an artifact created by a three\u2010dimensional structure being reduced to a two\u2010dimensional monolayer image. The shared attachments were independent of both the presence of link proteins and changes in spacing between proteoglycans, suggesting a proteoglycan\u2014proteoglycan interaction occurring before aggregation. The interactions were not influenced by proteoglycan concentration at the time of aggregation. Link proteins, however, did increase the number of proteoglycans on the aggregate that could be cross\u2010linked with a bifunctional reagent, suggesting that link proteins facilitate proteoglycan\u2014proteoglycan interactions.<\/jats:p>","DOI":"10.1002\/jor.1100090602","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T12:04:40Z","timestamp":1109246680000},"page":"777-786","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evidence of proteoglycan\/proteoglycan interactions within aggregates"],"prefix":"10.1002","volume":"9","author":[{"given":"Anthony","family":"Kahn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence A.","family":"Pottenger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank M.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall W.","family":"Viola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(62)90095-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2280077"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"958","DOI":"10.2106\/00004623-198365070-00011","article-title":"The structure of human chondrosarcoma proteoglycans","volume":"65","author":"Buckwalter JA","year":"1983","journal-title":"J Bone Joint Surg [Am]"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"9830","DOI":"10.1016\/S0021-9258(18)33848-1","article-title":"Electron microscopic studies of cartilage proteoglycans","volume":"257","author":"Buckwalter JA","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0174-173X(83)80028-4"},{"key":"e_1_2_1_7_2","first-page":"5361","article-title":"The effect of link protein on proteoglycan aggregate structure","volume":"259","author":"Buckwalter JA","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100070121"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02015399"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"17757","DOI":"10.1016\/S0021-9258(18)45444-0","article-title":"Complete primary structure of rat cartilage core protein deduced from cDNA clones","volume":"262","author":"Doege K","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2610801"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1770237"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(72)90160-2"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1350905"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"4232","DOI":"10.1016\/S0021-9258(19)42507-6","article-title":"Aggregation of cartilage proteoglycans. 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