{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:35:55Z","timestamp":1791232555936,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":3031,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[1996,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In total joint arthroplasty, long\u2010term outcomes depend in part on the biocompatibility of implant alloys. This study analyzed effects of surface finish and diamond\u2010like carbon coating on osteoblast cell adhesion to polished titanium\u2010aluminum\u2010vanadium and polished or grit\u2010blasted cobalt\u2010chromium\u2010molybdenum alloys. Osteoblast binding was tested in the presence and absence of the cell adhesion proteins fibronectin, laminin, fibrinogen, and vitronectin and was quantified by measurement of DNA content. Although adherence occurred in serum\u2010free medium, maximal osteoblast binding required serum and was similar for titanium and cobalt alloys at 2 and 12 hours. With the grit\u2010blasted cobalt alloy, cell binding was reduced 48% (p &lt; 0.05) by 24 hours. Coating the alloys with diamond\u2010like carbon did not alter osteoblast adhesion, whereas fibronectin pretreatment increased cell binding 2.6\u2010fold (p &lt; 0.05). In contrast, fibrinogen, vitronectin, and laminin did not enhance cell adhesion. These results support the hypothesis that cell adhesion proteins can modify cell binding to orthopaedic alloys. Although osteoblast binding was not affected by the presence of diamond\u2010like carbon, this coating substance may influence other longer term processes, such as bone formation, and deserves further study.<\/jats:p>","DOI":"10.1002\/jor.1100140605","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T11:09:44Z","timestamp":1109243384000},"page":"871-877","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Osteoblast adhesion to orthopaedic implant alloys: Effects of cell adhesion molecules and diamond\u2010like carbon coating"],"prefix":"10.1002","volume":"14","author":[{"given":"Roger","family":"Kornu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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