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In op\/op mice, the heterotopic bone consisted of a disorganized, densely packed mixture of irregular calcified cartilage, osleoid, Chondro\u2010osteoid, and fibrous tissue. Injections of recombinant human macrophage colony\u2010stimulating factor initiated bone resorption that began in the peripheral vascularized regions of the metaphyses and continued in central areas of uncalficified avascular chondro\u2010osteoid. On vascularized surfaces, osteoclasts were stained with tartrate\u2010resistant acid phosphatase. In op\/op mice treated with macrophage colony\u2010stimulating factor, the osteoclasts were small, with only two or three nuclei and they did not exhibit tartrate\u2010resistant acid phosphatase staining. In untreated op\/op mice, surgical blood clots persisted in the heterotopic sites as late as 3 weeks after the operation, whereas in treated op\/op mice, the blood clots were absorbed almost as rapidly as in normal mice. Histologically, recombinant human macrophage colony\u2010stimulating factor restored normal macrophage functions: absorption and organization of blood clot, osteoclastogenesis. synthesis of tartrate\u2010resistant acid phosphatase, bone remodeling, islands of myelopoiesis, and construction of an ossicle complete with a cortex and a medulla filled with functioning hematopoietic bone marrow.<\/jats:p>","DOI":"10.1002\/jor.1100150320","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T10:47:47Z","timestamp":1109242067000},"page":"456-462","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Treatment with recombinant human macrophage colony\u2010stimulating factor resorbs blood clot and restores osteoclastogenesis in heterotopic bone induced by partially purified native bone morphogenetic protein in osteopetrotic (op\/op) 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