{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:27:03Z","timestamp":1760887623443},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":2727,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Massive cortical bone allografts have been found to incorporate slowly into host bone and thus are subject to complications such as nonunion, fatigue fracture, and infection. To better understand and improve the process of osteoinduction in these types of bone grafts, a new experimental model was developed with use of diaphyseal cortical bone grafts from rat tibiae that were prepared by partial mineralization and drilling of 0.33 mm diameter holes with a pulsed, 2.94 \u03bcm wavelength, erbiunr:yttrium\u2010aluminum\u2010garnet laser. Six types of grafts were analyzed: untreated (Type I), demineralized 25 \u03bcm deep (Type II), demineralized 150 \u03bcm deep (Type III), laser perforated (Type V), laser perforated and then demineralized 25 \u03bcm deep (Type V), and laser perforated and then demineralized 150 \u03bcm deep (Type VI). The graft was orthotopically transplanted in the tibia of an adult Sprague\u2010Dawley rat and followed for as long as 4 months. Histologic evaluation at 1 and 4 months postoperatively with use of hematoxylin and eosin staining confirmed that there was new bone growth in Types II, III, V, and VI grafts. The amount of growth was estimated by comparing bone mineral density before implantation with values obtained after retrieval of the graft. These measurements were correlated to histomorphometric analysis of graft incorporation. The results show that the processes of partial demineralization (p &lt; 0.000001) and laser perforation with partial demineralization (p &lt; 0.000001) were both significant in enhancing bone growth in this model. New bone growth was significantly increased when the grafts were prepared with extensive demineralization (p &lt; 0.015). This study demonstrates that osteogenesis in cortical bone grafts can be fostered through the process of partial demineralization and laser perforation. To the extent that minimal partial demineralization and laser perforation allow maintenance of Structural integrity while altering the osteoinductive properties in such a way as to promote ingrowth of new bone, this experimental model represents an advance in understanding how osteogenesis in cortical bone grafts may be improved.<\/jats:p>","DOI":"10.1002\/jor.1100150518","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T10:47:57Z","timestamp":1109242077000},"page":"748-756","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Improved osteoinduction of cortical bone allografts: A study of the effects of laser perforation and partial demineralization"],"prefix":"10.1002","volume":"15","author":[{"given":"Kai\u2010Uwe","family":"Lewandrowski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William W.","family":"Tomford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin T.","family":"Schomacker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas F.","family":"Deutsch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry J.","family":"Mankin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/07853899309147303"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100070102"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199072060-00005"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199173080-00002"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820200603"},{"key":"e_1_2_1_7_2","unstructured":"GendlerE: Cartilage and bone induction by artificially perforated organic bone matrix. 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