{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T08:20:02Z","timestamp":1758874802029},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T00:00:00Z","timestamp":1159488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to explore the early treatment potential of anterior cruciate ligament (ACL) injuries using artificial juxtacrine stimulation by photo\u2010immobilization of a growth factor. A photo\u2010reactive epidermal growth factor complex (EGF\u2010Az) was synthesized by conjugating EGF with <jats:italic>N<\/jats:italic>\u2010(4\u2010azidobenzoyloxy) succinimide followed by immobilization onto polystyrene culture plates using UV irradiation. ACL cells from human tissues (1\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>cells, 100 \u00b5l\/well) were cultured as follows: control, no EGF; 50 \u00b5l native EGF; 50 \u00b5l EGF\u2010Az immobilized; and 100 \u00b5l EGF\u2010Az immobilized. The ACL cells were cultured long\u2010term and evaluated for possible differences in their responses to EGF. An in vitro wound closure assay was developed to enable examination of cellular proliferation and migration. ACL cell proliferation was most evident in the photo\u2010immobilized EGF culture group and was seen to increase in proportion to the amount of added EGF. In the in vitro wound closure assay, the lesioned area at 72 h after culture initiation was indistinguishable in the photo\u2010immobilized cultures, but remained clearly visible in the controls. We conclude that photo\u2010immobilized EGF induced rapid proliferation of ACL fibroblast cells by artificial juxtacrine stimulation and speculate that similar EGF immobilization onto bioabsorbable material (e.g., polyglycolic acid or polylactic acid) might contribute to a new therapy for the treatment of ACL injuries. \u00a9 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:73\u201380, 2007<\/jats:p>","DOI":"10.1002\/jor.20266","type":"journal-article","created":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T18:28:30Z","timestamp":1159554510000},"page":"73-80","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Proliferation of anterior cruciate ligament cells in vitro by photo\u2010immobilized epidermal growth factor"],"prefix":"10.1002","volume":"25","author":[{"given":"Young Kyun","family":"Woo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soon Yong","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwa Sung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong 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