{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:15:04Z","timestamp":1787746504486,"version":"build-2784847793"},"reference-count":19,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,6,13]],"date-time":"2007-06-13T00:00:00Z","timestamp":1181692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently developed in vivo animal high\u2010resolution micro\u2010CT scanners offer the possibility to monitor longitudinal changes in bone microstructure of small rodents, but may impose high radiation doses that could damage bone tissue. The goal of this study was to determine the effects on the bone of 8 weeks of in vivo scanning of the proximal tibia in female Wistar rats. Eight weekly CT scans were made of the right proximal tibia of nine female, 30\u2010week\u2010old, retired\u2010breeder, Wistar rats. Two weeks after the last weekly scan, a final scan was made. The left leg was only scanned during first and final measurements and served as a control. A two\u2010way ANOVA with repeated measures was performed on the first and last measurements of left and right tibiae for six bone structural parameters. Bone marrow cells were flushed out and tested for cell viability. No significant difference was found between left and right for any of the bone structural parameters (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). Structure model index and trabecular separation significantly changed as a result of aging, while none of the other parameters did. No significant difference was found between left and right in absolute and percentage number of cell viability. We did not find any indication that the applied scanning regime, in combination with the particular settings used, would affect the results of in vivo bone structural measurements in long\u2010term studies using aged, female Wistar rats. However, careful consideration should be made when determining the number of scans, particularly when a different experimental design is used. \u00a9 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1325\u20131332, 2007<\/jats:p>","DOI":"10.1002\/jor.20439","type":"journal-article","created":{"date-parts":[[2007,6,14]],"date-time":"2007-06-14T04:39:25Z","timestamp":1181795965000},"page":"1325-1332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["No effects of in vivo micro\u2010CT radiation on structural parameters and bone marrow cells in proximal tibia of wistar rats detected after eight weekly scans"],"prefix":"10.1002","volume":"25","author":[{"given":"Julienne E.M.","family":"Brouwers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert","family":"van Rietbergen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rik","family":"Huiskes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,9,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2003.08.012"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2003.18.9.1622"},{"key":"e_1_2_1_4_2","first-page":"310","article-title":"Longitudinal micro\u2010CT scans to evaluate bone architecture","volume":"5","author":"Waarsing JH","year":"2005","journal-title":"J Musculoskelet Neuronal Interact"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.040705"},{"key":"e_1_2_1_6_2","article-title":"The essential physics of medical imaging","author":"Bushberg JT","year":"2005","journal-title":"Baltimore: Williams and Wilkins."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/02844318509074508"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1148\/26.1.56"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760020601"},{"key":"e_1_2_1_10_2","unstructured":"JudexS ChungH TorabA et al.Micro\u2010CT induced radiation does not exacerbate disuse related bone loss. In: Transactions of the 51stannual meeting of the ORS. Washington DC february 2005. Poster no 1546."},{"key":"e_1_2_1_11_2","volume-title":"The effects of radiation on ovariectomized mice as a function of genetic strain by in vivo micro\u2010computed tomography","author":"Klinck RJ","year":"2006"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/02841868309134352"},{"key":"e_1_2_1_13_2","first-page":"1","article-title":"Effects of irradiation on growing bones","volume":"45","author":"Engstrom H","year":"1987","journal-title":"Swed Dent J Suppl"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2003.12.036"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20063"},{"key":"e_1_2_1_16_2","unstructured":"WaarsingJH.2006.Exploring bone dynamics by in\u2010vivo micro\u2010CT imaging (dissertation). Erasmus Medical Center Rotterdam The Netherlands. This is a dissertation."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2006.04.017"},{"key":"e_1_2_1_18_2","first-page":"211","article-title":"Comparative effects of orchidectomy and sciatic neurectomy on cortical and cancellous bone in young growing rats","volume":"21","author":"Iwamoto J","year":"2003","journal-title":"J Bone Miner Metab"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.132.4.8462456"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001980200113"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.20439","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.20439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T05:21:47Z","timestamp":1700025707000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.20439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,13]]},"references-count":19,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1002\/jor.20439"],"URL":"https:\/\/doi.org\/10.1002\/jor.20439","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,13]]},"assertion":[{"value":"2006-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-09-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}