{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:42:50Z","timestamp":1761100970203},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,3,7]],"date-time":"2008-03-07T00:00:00Z","timestamp":1204848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Impaired function of shoulder muscles, resulting from rotator cuff tears, is associated with abnormal deposition of fat in muscle tissue, but corresponding cellular and molecular mechanisms, likely reflected by altered gene expression profiles, are largely unknown. Here, an analysis of muscle gene expression was carried out by semiquantitative RT\u2010PCR in total RNA extracts of supraspinatus biopsies collected from 60 patients prior to shoulder surgery. A significant increase of \u03b1\u2010skeletal muscle actin (<jats:italic>p<\/jats:italic>\u2009=\u20090.0115) and of myosin heavy polypeptide 1 (<jats:italic>p<\/jats:italic>\u2009=\u20090.0147) gene transcripts was observed in parallel with progressive fat deposition in the muscle, assessed on parasagittal T1\u2010weighted turbo\u2010spin\u2010echo magnetic resonance images according to Goutallier. Upregulation of \u03b1\u2010skeletal muscle actin and of myosin heavy polypeptide\u20101 has been reported to be associated with increased muscle tissue metabolism and oxidative stress. The findings of the present study, therefore, challenge the hypothesis that increased fat deposition in rotator cuff muscle after injury reflects muscle degeneration. \u00a9 2008 Orthopaedic Research Society. 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