{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:41:17Z","timestamp":1791304877532,"version":"4.3.1"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,3,11]],"date-time":"2010-03-11T00:00:00Z","timestamp":1268265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Susceptibility to osteolysis after total hip arthroplasty (THA) varies between individuals. We examined whether patients susceptible to osteolysis (group I, <jats:italic>n<\/jats:italic>\u2009=\u200934 subjects) after cemented Charnley THA have quantitatively different innate immune responses to pro\u2010inflammatory stimuli versus patients without this susceptibility (group II, <jats:italic>n<\/jats:italic>\u2009=\u200928 subjects) at a mean of 14 years after primary surgery. Extracted peripheral blood mononuclear cells were stimulated for 3\u2009h using endotoxin (lipopolysaccharide\u2014LPS, 100\u2009ng\/mL), endotoxin\u2010stripped titanium particles (Ti) or endotoxin\u2010stripped particles with adherent LPS added\u2010back (TI\u2009+\u2009LPS). Subjects returned 1 week later and the experimental protocol was repeated. Assays for mRNA induction for interleukin (IL)\u20101\u03b1, IL\u20101\u03b2, IL\u20101Ra, IL\u20106, IL\u201010, IL\u201018, and tumor necrosis factor (TNF) were made using quantitative real\u2010time PCR. Although baseline levels of mRNA expression were slightly lower in group I, inducibility of mRNA expression was markedly greater in group I versus group II for all cytokines in response to LPS or Ti\u2009+\u2009LPS, and for IL\u20101\u03b1 in response to Ti (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). LPS or Ti\u2009+\u2009LPS stimulation also resulted in an increase in the IL\u20101\/IL\u20101Ra mRNA ratio in group I versus group II (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). mRNA induction was highly reproducible between subject visits (<jats:italic>r<\/jats:italic>\u2009&gt;\u20090.7, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Osteolysis\u2010susceptible patients show repeatable, quantitatively different patterns of innate cytokine gene expression in response to pro\u2010inflammatory stimuli versus THA patients who do not display this susceptibility. These innate immune differences may contribute to the variation in osteolysis\u2010susceptibility observed clinically between individuals. \u00a9 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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