{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T22:57:30Z","timestamp":1776725850222,"version":"3.51.2"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,8,25]],"date-time":"2010-08-25T00:00:00Z","timestamp":1282694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Toll\u2010like receptors (TLRs) are involved in mediating cell activation on stimulation with microbial components. Our objective was to investigate the role of TLR\u20102 mediated by the NF\u2010\u03baB pathway in septic arthritic chondrocytes. TLR\u20101, \u20102, and \u20106 mRNA expression levels were investigated in septic and normal chondrocytes using real\u2010time reverse transcription\u2010PCR. TLR\u20102 and MMP\u201013 mRNA and protein levels were measured using real\u2010time PCR and Western blot analysis, respectively. Blocking TLR\u20102 mRNA expression was performed using small interfering RNA (siRNA) against TLR\u20102 and subsequently MMP\u20103, MMP\u201013, IL\u20101\u03b2, and IL\u20106 mRNA levels, as well as p65 NF\u2010\u03baB, IkB\u03b1, and MMP\u201013 protein levels were evaluated using real\u2010time PCR and Western blot analysis. IL\u20106 protein levels were measured using ELISA assay. We observed that TLR\u20101, \u20102, and \u20106 mRNA expression levels were significantly higher in septic compared to normal chondrocytes. MMP\u201013 mRNA and protein expressions were also significantly upregulated in septic arthritic cartilage. Blocking TLR\u20102 mRNA expression in septic chondrocytes resulted in significant increase of inactivated nonphosphorylated p65 NF\u2010\u03baB and IkB\u03b1 protein levels and reduction in MMP\u201013, IL\u20101\u03b2, and IL\u20106 expression. Our findings suggest the pro\u2010inflammatory and catabolic role of TLR\u20102 mediated by the NF\u2010\u03baB pathway in septic arthritis. Modulation of TLR\u2010mediated signaling may be a potential therapeutic strategy for the prevention of postinfectious cartilage degradation in articular joints. \u00a9 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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