{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:47:14Z","timestamp":1789951634174,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Management of various tumor metastases to bone has dramatically improved, but this is not so for renal cell carcinoma (RCC), which is a difficult surgical problem due to its great vascularity. Furthermore, the unique mechanisms that mediate RCC vasculogenesis in bone remain unknown. To understand this process we developed a xenograft model that recapitulates highly vascular RCC versus less vascular tumors that metastasize to bone. Human tumor cell lines of RCC (786\u2010O), prostate cancer (PC3), lung cancer (A549), breast cancer (MDA\u2010MB231), and melanoma (A375) were transduced with firefly luciferase (Luc), injected into the tibiae of nude mice, and differences in growth, osteolysis, and vascularity were assessed by longitudinal bioluminescent imaging, micro\u2010CT for measurement of calcified tissues and vascularity and histology. The results showed that while RCC\u2010Luc has reduced growth and osteolytic potential versus the other tumor lines, it displayed a significant increase in vascular volume (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). This expansion was due to 3\u2010 and 5\u2010fold increases in small and large vessel numbers respectively. In vitro gene expression profiling revealed that RCC\u2010Luc expresses significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) more <jats:italic>vegf\u2010a<\/jats:italic> (10\u2010fold) and 20\u2010 to 30\u2010fold less <jats:italic>ang\u20101<\/jats:italic> versus the other lines. These data demonstrate the utility of this model to study the unique vasculogenic properties of RCC bone metastases. \u00a9 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:325\u2013333, 2012<\/jats:p>","DOI":"10.1002\/jor.21500","type":"journal-article","created":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T19:43:58Z","timestamp":1312227838000},"page":"325-333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Unique angiogenic and vasculogenic properties of renal cell carcinoma in a xenograft model of bone metastasis are associated with high levels of <i>vegf\u2010a<\/i> and decreased <i>ang\u20101<\/i> expression"],"prefix":"10.1002","volume":"30","author":[{"given":"Chao","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward M.","family":"Schwarz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik R.","family":"Sampson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robinder 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