{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T11:40:30Z","timestamp":1779363630241,"version":"3.53.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T00:00:00Z","timestamp":1311811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>ADAMTS5 (aggrecanase\u20102) is an extracellular matrix\u2010degrading protease implicated in cartilage destruction in arthritis. Our goals were to determine expression sites of <jats:italic>Adamts5<\/jats:italic> in the murine musculoskeletal system and in an ex vivo joint inflammation model. In mice with an intragenic <jats:italic>LacZ<\/jats:italic> reporter controlled by the <jats:italic>Adamts5<\/jats:italic> promoter, \u03b2\u2010galactosidase staining was used to identify <jats:italic>Adamts5<\/jats:italic> expressing cells. Mice expressing one wild\u2010type <jats:italic>Adamts5<\/jats:italic> allele were used to determine distribution of <jats:italic>Adamts5<\/jats:italic> mRNA, cleaved aggrecan and versican, and the ADAMTS5 activating enzymes furin and PACE4. Quantitative RT\u2010PCR and immunoblotting were used to validate the immunohistochemistry results. <jats:italic>Adamts5<\/jats:italic> was expressed in mouse synovium, tenosynovium, bone marrow sinusoids, tendons, ligaments, ligament insertions, periosteal cells, and bone vasculature. In knee joint explants treated with IL\u20101\u03b1 and TNF\u03b1, <jats:italic>Adamts5<\/jats:italic> expression was induced in tenocytes, synovium, and in patellar, but not femoral or tibial articular cartilage. In contrast, increased proteoglycan breakdown in tibial and femoral articular cartilage was associated with increased immunohistochemical staining of PACE4 and furin. These studies identify diverse cell types in the musculoskeletal system that express <jats:italic>Adamts5<\/jats:italic>. They also suggest that <jats:italic>Adamts5<\/jats:italic> induction in joint components other than cartilage, and its post\u2010translational activation by PACE4 and\/or furin may be important in the pathophysiology of arthritis. \u00a9 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:226\u2013233, 2012<\/jats:p>","DOI":"10.1002\/jor.21508","type":"journal-article","created":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T15:25:45Z","timestamp":1311866745000},"page":"226-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<i>Adamts5<\/i> (aggrecanase\u20102) is widely expressed in the mouse musculoskeletal system and is induced in specific regions of knee joint explants by inflammatory cytokines"],"prefix":"10.1002","volume":"30","author":[{"given":"James D.","family":"Wylie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason C.","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"McCulloch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suneel S.","family":"Apte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,7,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.33.23443"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.10.008"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290106"},{"key":"e_1_2_6_5_2","first-page":"881","article-title":"Divide, accumulate, differentiate: Cell condensation in skeletal development revisited","volume":"39","author":"Hall BK","year":"1995","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03369"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03417"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI30765"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.07.012"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701523200"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21215"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0909"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2008.09.018"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2009.02.006"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_16_2","first-page":"971","article-title":"Beta\u2010galactosidase staining on bone marrow. The osteoclast pitfall","volume":"22","author":"Kopp HG","year":"2007","journal-title":"Histol Histopathol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.1199"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21332"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0838.2009.00938.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3500181"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2004.03.004"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(97)80042-8"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2010.00140.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2009.07.006"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.01990.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199802)41:2<274::AID-ART11>3.0.CO;2-Z"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.028"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3540689"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3210587"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313151200"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211009200"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2005.09.018"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21022"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20558"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.21508","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.21508","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.21508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T20:25:43Z","timestamp":1696883143000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.21508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,28]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1002\/jor.21508"],"URL":"https:\/\/doi.org\/10.1002\/jor.21508","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7,28]]},"assertion":[{"value":"2011-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-06-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-07-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}