{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:41:20Z","timestamp":1791304880565,"version":"4.3.1"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,9,12]],"date-time":"2011-09-12T00:00:00Z","timestamp":1315785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biological mechanisms leading to periprosthetic osteolysis involve both chemokines and the monocyte\/macrophage cell lineage. Whether MCP\u20101 plays a major role in macrophage recruitment in the presence of wear particles is unknown. We tested two hypotheses: (1) that exogenous local delivery of MCP\u20101 induces systematic macrophage recruitment and (2) that blockade of the MCP\u20101 ligand\u2010receptor axis decreases macrophage recruitment and osteolysis in the presence of ultra high molecular weight polyethylene (UHMWPE) particles. Six groups of nude mice were used. We used non\u2010invasive imaging to assay macrophage recruitment and osteolysis. A murine macrophage cell line and primary wild type and CCR2 knockout murine macrophages were used as the reporter cells. Particles were infused into the femoral canal. Bioluminescence and immunohistochemical staining were used to confirm the migration of reporter cells. Locally infused MCP\u20101 induced systemic macrophage trafficking to bone. Injection of MCP\u20101 receptor antagonist significantly decreased reporter cell recruitment to bone infused with UHMWPE particles and decreased osteolysis. Systemic migration of reporter cells to infused particles was decreased when the reporter cells were deficient in the CCR2 receptor. Interruption of the MCP\u20101 ligand\u2010receptor axis appears to be a viable strategy to mitigate trafficking of macrophages and osteolysis due to UHMWPE particles. \u00a9 2011 Orthopaedic Research Society. \u00a9 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:547\u2013553, 2012<\/jats:p>","DOI":"10.1002\/jor.21548","type":"journal-article","created":{"date-parts":[[2011,9,12]],"date-time":"2011-09-12T17:19:18Z","timestamp":1315847958000},"page":"547-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Selective inhibition of the MCP\u20101\u2010CCR2 ligand\u2010receptor axis decreases systemic trafficking of macrophages in the presence of UHMWPE 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