{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T12:25:57Z","timestamp":1766492757210},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,4,16]],"date-time":"2012-04-16T00:00:00Z","timestamp":1334534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have found that microRNA\u201021 (miR\u201021) is an important functional factor during osteoclast differentiation. Abnormal osteoclastogenesis induced by wear particles is the main cause of aseptic loosening in joint replacements. The aim of the present study is to investigate the possible role of miR\u201021 in the pathogenesis of particle\u2010induced osteolysis (PIO). miR\u201021 expression was examined in a PIO mouse model using real\u2010time (RT\u2010PCR). Osteoclastogenesis was determined by a tartrate resistant acid phosphatase (TRAP) quantification method. A toluidine blue staining assay was used to examine calvarial osteolysis. The results demonstrated that miR\u201021 was significantly upregulated in the PIO animal model. Knocking out miR\u201021 in the particle\u2010stimulated tissue could ameliorate osteolysis symptoms. Additionally, through our analysis of PDCD4 and AP\u20101 expression, we suggest that the feedback loop of AP\u20101, miR\u201021, and PDCD4 might have an important influence on the development of PIO and that miR\u201021 is a potential target for implant loosening therapies. \u00a9 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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