{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T05:32:56Z","timestamp":1770355976777,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,14]],"date-time":"2012-09-14T00:00:00Z","timestamp":1347580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Matrix metalloproteinases (MMPs) are expressed in Dupuytren's contracture and play a role in matrix remodeling. We tested the role of tension on contractility and MMP expression in Dupuytren's nodule and cord cells. Cells were subjected to pre\u2010determined loading patterns of known repeatable magnitudes (static load, unloading, and overloading) and tested for MMP gene expression (MMP\u20101, \u20102, \u20109, \u201013, and TIMP\u20101, \u20102) and force generation using a tension\u2010culture force monitor. Matrix remodeling was assessed by addition of cytochalasin D and residual matrix tension was quantified. Nodule compared to cord and control cells demonstrate greater force generation and remodeling (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Nodule cells subjected to a reduced load and overloading led to threefold increase of MMP\u20101, \u20102, and \u20109 compared to static load, whilst cord and control cells only showed a twofold increase of MMP\u20109. Nodule cells subjected to overloading showed a twofold increase in TIMP\u20102 expression, whilst cord and control cells showed a twofold increase in TIMP\u20101 expression. Nodule cells differ from cord cells by increased force generation in response to changes in the mechanical environment and related MMP\/TIMP\u2010mediated matrix remodeling. In turn this may lead to permanent matrix shortening and digital contracture. Interventional therapies should be aimed at nodule cells to prevent contraction and subsequent permanent matrix remodeling. \u00a9 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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