{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:01:45Z","timestamp":1791406905136,"version":"4.3.3"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,2,25]],"date-time":"2013-02-25T00:00:00Z","timestamp":1361750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Hypothalamo\u2010pituitary disconnection (HPD) leads to low bone turnover and osteoporosis in sheep. To determine the sustainability of bone loss and its biomechanical relevance, we studied HPD\u2010sheep 24 months after surgery (<jats:italic>HPD<\/jats:italic>\u2009+\u2009<jats:italic>OVX\u201024<\/jats:italic>) in comparison to untreated control (<jats:italic>Control<\/jats:italic>), ovariectomized sheep (<jats:italic>OVX<\/jats:italic>), and sheep 12 months after HPD (<jats:italic>HPD<\/jats:italic>\u2009+\u2009<jats:italic>OVX\u201012<\/jats:italic>). We performed histomorphometric, HR\u2010pQCT, and qBEI analyses, as well as biomechanical testing of all ewes studied. Twenty\u2010four months after HPD, histomorphometric analyses of the iliac crest showed a significant reduction of BV\/TV by 60% in comparison to <jats:italic>Control<\/jats:italic>. Cortical thickness of the femora measured by HR\u2010pQCT did not change between 12 and 24 months after HPD but remained decreased by 30%. These structural changes were caused by a persisting depression of osteoblast and osteoclast cellular activity. Biomechanical testing of the femora showed a significant reduction of bending strength, whereas calcium content and distribution was found to be unchanged. In conclusion, HPD surgery leads to a persisting low turnover status with negative turnover balance in sheep followed by dramatic cortical and trabecular bone loss with consequent biomechanical impairment. \u00a9 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. 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