{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:57:57Z","timestamp":1792735077054,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>We have previously reported the high regenerative potential of murine muscle\u2010derived stem cells (mMDSCs) that are capable of differentiating into multiple mesodermal cell lineages, including myogenic, endothelial, chondrocytic, and osteoblastic cells. Recently, we described a putative human counterpart of mMDSCs, the myogenic endothelial cells (MECs), in adult human skeletal muscle, which efficiently repair\/regenerate the injured and dystrophic skeletal muscle as well as the ischemic heart in animal disease models. Nevertheless it remained unclear whether human MECs, at the clonal level, preserve mMDSC\u2010like chondrogenic and osteogenic potentials and classic stem cell characteristics including high proliferation and resistance to stress. Herein, we demonstrated that MECs, sorted from fresh postnatal human skeletal muscle biopsies, can be grown clonally and exhibit robust resistance to oxidative stress with no tumorigeneity. MEC clones were capable of differentiating into chondrocytes and osteoblasts under inductive conditions in vitro and participated in cartilage and bone formation in vivo. Additionally, adipogenic and angiogenic potentials of clonal MECs (cMECs) were observed. Overall, our study showed that cMECs not only display typical properties of adult stem cells but also exhibit chondrogenic and osteogenic capacities in vitro and in vivo, suggesting their potential applications in articular cartilage and bone repair\/regeneration. \u00a9 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1089\u20131095, 2013<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.22335","type":"journal-article","created":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T12:30:41Z","timestamp":1364819441000},"page":"1089-1095","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Human myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level"],"prefix":"10.1002","volume":"31","author":[{"given":"Bo","family":"Zheng","sequence":"first","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangheng","family":"Li","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.W.","family":"Chen","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Bioengineering University of Pittsburgh Swanson School of Engineering Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget M.","family":"Deasy","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania"},{"name":"Department of Bioengineering University of Pittsburgh Swanson School of Engineering Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan B.","family":"Pollett","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Sun","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Pediatrics Children's Hospital of Pittsburgh of UPMC Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Drowley","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burhan","family":"Gharaibeh","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania"},{"name":"Department of Bioengineering University of Pittsburgh Swanson School of Engineering Pittsburgh, Pennsylvania"},{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvydas","family":"Usas","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"P\u00e9ault","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Pediatrics Children's Hospital of Pittsburgh of UPMC Pittsburgh, Pennsylvania"},{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, Pennsylvania"},{"name":"David Geffen School of Medicine University of California at Los Angeles Los Angeles, California"},{"name":"Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine University of Edinburgh Edinburgh United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnny","family":"Huard","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Bridgeside Point II, Suite 206, 450 Technology Drive Pittsburgh, Pennsylvania 15219"},{"name":"Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania"},{"name":"McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.sj.6300145"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.4.869"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5356.1528"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082254"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1542"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.07.003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1508"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.5.1085"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200108150"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302521"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.142"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21632"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1334"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2008.07.064"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.050513"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0214"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.1891"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368909X471305"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1287"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X636876"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-08-0693"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.11.2773"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2003.08.001"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05282"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.12.012"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.stem.2010.01.011","article-title":"Stem cells and the niche: a dynamic duo","volume":"6","author":"Voog J","journal-title":"Cell Stem Cell"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/597439"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.22335","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.22335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T04:33:00Z","timestamp":1698640380000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.22335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4]]},"references-count":28,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/jor.22335"],"URL":"https:\/\/doi.org\/10.1002\/jor.22335","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4]]},"assertion":[{"value":"2012-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}