{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T16:34:18Z","timestamp":1784219658468,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,30]],"date-time":"2013-11-30T00:00:00Z","timestamp":1385769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Faculty research from Yonsei University College of Medicine","award":["6-2010-0612"],"award-info":[{"award-number":["6-2010-0612"]}]},{"name":"The Brain Korea 21 PLUS Project for Medical Science, Yonsei University"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Dupuytren's disease is a fibroproliferative connective tissue disorder characterized by contracture of the palmer fascia of the hand. Relaxin (RLN) is a multifunctional factor which contributes to the remodeling of the pelvic ligament by inhibiting fibrosis and inflammatory activities. The aim of this study was to investigate the effect of the RLN gene on the inhibition of fibrosis in myofibroblastic cells. Myofibroblast cells with adenovirus LacZ (Ad\u2010LacZ) as a marker gene or adenovirus relaxin (Ad\u2010RLN) as therapeutic gene showed transgene expressions in beta\u2010galactosidase assay and Western blot analysis. Myofibroblastic cells with Ad\u2010RLN demonstrated a 22% and 48% reduction in collagen I and III mRNA expressions respectively, a 50% decrease in MMP\u20101, 70% decrease in MMP\u20102, 80% decrease in MMP\u20109, and a 15% reduction in MMP\u201013 protein expression compared with cultures with viral control and saline control. In addition, myofibroblastic cells with Ad\u2010RLN showed a 40% decrease in TIMP 1 and a 15% increase in TIMP 3 protein expression at 48\u2009h compared to cultures with viral control and saline control. Also, myofibroblastic cell with Ad\u2010RLN demonstrated a 74% inhibition of fibronectin and a 52% decrease in total collagen synthesis at 48\u2009h compared with cultures with viral control and saline control. In conclusion, the RLN gene render antifibrogenic effect on myofibroblastic cells from Dupuytren's nodule via direct inhibition of collagen synthesis not through collagenolytic pathway such as MMP\u20101, \u201013, TIMP 1, and 3. Therefore relaxin can be an alternative therapeutic strategy in initial stage of Dupuytren's disease by its antifibrogenic effect. \u00a9 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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