{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:59:41Z","timestamp":1792799981820,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,7]],"date-time":"2014-03-07T00:00:00Z","timestamp":1394150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"US DHHS, National Institutes of Health\/NIAMS","award":["P50 AR055533"],"award-info":[{"award-number":["P50 AR055533"]}]},{"name":"Merit Review Award from the Department of Veterans Affairs"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Post\u2010traumatic osteoarthritis (PTOA) is characterized by progressive cartilage degeneration in injured joints. Since fibronectin\u2010fragments (Fn\u2010fs) degrade cartilage mainly through up\u2010regulating matrix metalloproteinases (MMPs) and pro\u2010inflammatory cytokines, we hypothesized that Fn\u2010fs play a key role in PTOA by promoting chondrolysis in and around injured cartilage. To test this hypothesis, we profiled the catabolic events focusing on fibronectin fragmentation and proteinase expression in bovine osteochondral explants following a single blunt impact on cartilage with a drop tower device which created partial\u2010thickness tissue damage. Injured and control explants were cultured for up to 14 days. The presence of Fn\u2010fs, MMPs (\u20101, \u20103, \u201013), ADAMTS\u20105 in culture media and in cartilage was determined with immunoblotting. The daily proteoglycan (PG) depletion of cartilage matrix was assessed with DMMB assay. The effect of explant\u2010conditioned media on chondrocytes was also examined with immunoblotting. Impacted cartilage released significantly higher amount of native Fn, three chondrolytic Fn\u2010fs and PG than non\u2010impacted controls did. Those increases coincided with up\u2010regulation of MMP\u20103 both in culture media and in impacted cartilage. These findings support our hypothesis that PTOA may be propelled by Fn\u2010fs which act as catabolic mediators through up\u2010regulating cartilage\u2010damaging proteinases. \u00a9 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:811\u2013818, 2014.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jor.22610","type":"journal-article","created":{"date-parts":[[2014,3,7]],"date-time":"2014-03-07T07:06:41Z","timestamp":1394176001000},"page":"811-818","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["A single blunt impact on cartilage promotes fibronectin fragmentation and upregulates cartilage degrading stromelysin\u20101\/matrix metalloproteinase\u20103 in a bovine\n                    <i>ex vivo<\/i>\n                    model"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6993-8273","authenticated-orcid":false,"given":"Lei","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Orthopaedics and Rehabilitation University of Iowa 500 Newton Road, 1182 Medical Laboratories Iowa City Iowa 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